MiR-219a-1 Inhibits Colon Cancer Cells Proliferation and Invasion by Targeting

Overview

Journal Cancer Biol Ther

Specialties Oncology
Pharmacology

Date 2020 Nov 21

PMID 33218285

Citations 10

Authors

Keqing Xu

Jie Shi

Dongping Mo

Yanhua Yang

Qiang Fu

Ying Luo

Affiliations

Soon will be listed here.

Abstract

Colon cancer is the third most common cancer worldwide. Many miRNAs have been reported to be involved in colon cancer progression. However, there are only a few studies on the role of miR-219a-1 in colon cancer, and the molecular mechanisms involved remain unclear. The aim of this study was to investigate the miR-219a-1 level in patients with colon cancer and to explore both the effects and regulatory mechanisms of miR-219a-1 in the malignancy of colon cancer cells. Real-time PCR and western blot analysis were used to analyze the expression levels of miR-219a-1 and mediator of ErbB2-driven cell motility 1. Cell Counting Kit-8, transwell and wound-healing assays were performed to investigate the malignant ability of colon cancer cells. A luciferase assay was performed to explore whether miR-219a-1 could directly bind to 3'-UTR region of . miR-219a-1 was found to be downregulated in colon cancer cell lines and in patients with colon cancer. Additionally, miR-219a-1 could inhibit colon cancer cell proliferation, invasion and migration. We identified as a novel potential target gene of miR-219a-1. Luciferase assays showed that miR-219a-1 could directly bind to 3'-UTR of . Overexpression of miR-219a-1 in colon cancer cells could inhibit the expression of . Furthermore, was upregulated in patients with colon cancer, which was inversely correlated with miR-219a-1 levels. In conclusion, our study revealed that miR-219a-1 exerts anti-tumor effects and regulates colon cancer cell proliferation, invasion and migration by targeting , suggesting that miR-219a-1 could act as a therapeutic target in colon cancer.

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