Differential Requirements of Gag and Gamma-actin Domains for Transforming Potential of Gardner-Rasheed Feline Sarcoma Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1989 Mar 1

PMID 2536827

Citations 5

Authors

J Miyoshi

Y Miyoshi

H Sasai

N Sakai

T Katsumata

T KAKUNAGA

Affiliations

Soon will be listed here.

Abstract

The oncogene of Gardner-Rasheed feline sarcoma virus (GR-FeSV) encodes the 70-kilodalton protein containing gag(p15), gamma-actin, and fgr domains. To determine the role of these domains in the biological activity of P70gag-actin-fgr, we have constructed in-frame deletion and insertion mutants of GR-FeSV. We found, first, that the gamma-actin region could be deleted without affecting the transforming ability of these constructs, although an insertion mutant in the middle of the gamma-actin domain (map position 671) was partially defective in transformation and specifically had a reduced level of in vitro autophosphorylation activity. Second, mutations affecting the C-terminal third of the gag region appeared to abolish the ability to transform NIH 3T3 cells and autophosphorylation activity. These results suggest that the gamma-actin domain is not essentially required for the transforming activity of GR-FeSV but that it may take part in maintaining the conformational integrity of P70gag-actin-fgr and that the gag(p15) domain might have a critical role in modulating the function of P70gag-actin-fgr.

Citing Articles

Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours: detection of transforming genes in X-ray-related mouse tumours.

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Characterization of the transforming domain of a feline sarcoma virus encoding a fgr-related tyrosine kinase.

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Hamster cell line suitable for transfection assay of transforming genes.

Higashi T, Sasai H, Suzuki F, Miyoshi J, Ohuchi T, Takai S Proc Natl Acad Sci U S A. 1990; 87(7):2409-13.

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Role of gag sequence in the biochemical properties and transforming activity of the avian sarcoma virus UR2-encoded gag-ros fusion protein.

Jong S, Wang L J Virol. 1990; 64(12):5997-6009.

PMID: 2173777 PMC: 248773. DOI: 10.1128/JVI.64.12.5997-6009.1990.

Structure and transforming potential of the human cot oncogene encoding a putative protein kinase.

Miyoshi J, Higashi T, Mukai H, Ohuchi T, KAKUNAGA T Mol Cell Biol. 1991; 11(8):4088-96.

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