Characterization of the Transforming Domain of a Feline Sarcoma Virus Encoding a Fgr-related Tyrosine Kinase

Overview

Journal Arch Virol

Specialty Microbiology

Date 1994 Jan 1

PMID 8279950

Authors

G Weyrauch

A Barnekow

Affiliations

Soon will be listed here.

Abstract

We investigated the transforming domain of a recently isolated feline sarcoma virus (TP1-FeSV) which encodes a fgr-related tyrosine kinase expressed as a gag-fgr fusion protein. The gag portion was removed and replication-competent expression vectors (RCAS) with inserted v-fgr sequences were established. Chicken embryo fibroblasts (CEF) were transfected and monitored for replication, integration and transcription of the proviral constructs. We demonstrated that transfected cells display morphological changes and are able to form colonies in soft-agar. This suggests that the gag portion of the fusion protein from TP1-FeSV is not necessary for the transformation of fibroblasts.

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