Structural Basis for Ion Selectivity Revealed by High-resolution Crystal Structure of Mg2+ Channel MgtE

Overview

Journal Nat Commun

Specialty Biology

Date 2014 Nov 5

PMID 25367295

Citations 27

Authors

Hironori Takeda

Motoyuki Hattori

Tomohiro Nishizawa

Keitaro Yamashita

Syed T A Shah

Martin Caffrey

Andres D Maturana

Ryuichiro Ishitani

Osamu Nureki

Affiliations

Soon will be listed here.

Abstract

Magnesium is the most abundant divalent cation in living cells and is crucial to several biological processes. MgtE is a Mg(2+) channel distributed in all domains of life that contributes to the maintenance of cellular Mg(2+) homeostasis. Here we report the high-resolution crystal structures of the transmembrane domain of MgtE, bound to Mg(2+), Mn(2+) and Ca(2+). The high-resolution Mg(2+)-bound crystal structure clearly visualized the hydrated Mg(2+) ion within its selectivity filter. Based on those structures and biochemical analyses, we propose a cation selectivity mechanism for MgtE in which the geometry of the hydration shell of the fully hydrated Mg(2+) ion is recognized by the side-chain carboxylate groups in the selectivity filter. This is in contrast to the K(+)-selective filter of KcsA, which recognizes a dehydrated K(+) ion. Our results further revealed a cation-binding site on the periplasmic side, which regulate channel opening and prevents conduction of near-cognate cations.

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