Structural Basis for the Mg Recognition and Regulation of the CorC Mg Transporter

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2021 Feb 11

PMID 33568487

Citations 23

Authors

Yichen Huang

Fei Jin

Yosuke Funato

Zhijian Xu

Weiliang Zhu

Jing Wang

Minxuan Sun

Yimeng Zhao

Ye Yu

Hiroaki Miki

Motoyuki Hattori

Affiliations

Soon will be listed here.

Abstract

The CNNM/CorC family proteins are Mg transporters that are widely distributed in all domains of life. In bacteria, CorC has been implicated in the survival of pathogenic microorganisms. In humans, CNNM proteins are involved in various biological events, such as body absorption/reabsorption of Mg and genetic disorders. Here, we determined the crystal structure of the Mg-bound CorC TM domain dimer. Each protomer has a single Mg binding site with a fully dehydrated Mg ion. The residues at the Mg binding site are strictly conserved in both human CNNM2 and CNNM4, and many of these residues are associated with genetic diseases. Furthermore, we determined the structures of the CorC cytoplasmic region containing its regulatory ATP-binding domain. A combination of structural and functional analyses not only revealed the potential interface between the TM and cytoplasmic domains but also showed that ATP binding is important for the Mg export activity of CorC.

Citing Articles

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