The Long Noncoding RNA Neat1 is Required for Mammary Gland Development and Lactation

Overview

Journal RNA

Specialty Molecular Biology

Date 2014 Oct 16

PMID 25316907

Citations 107

Authors

Laura Standaert

Carmen Adriaens

Enrico Radaelli

Alexandra Van Keymeulen

Cedric Blanpain

Tetsuro Hirose

Shinichi Nakagawa

Jean-Christophe Marine

Affiliations

Soon will be listed here.

Abstract

The lncRNA Neat1 is an essential architectural component of paraspeckle nuclear bodies. Although cell-based studies identified Neat1-paraspeckles as key regulators of gene expression through retention of hyperdited mRNAs and/or transcription factors, it is unclear under which specific physiological conditions paraspeckles are formed in vivo and whether they have any biological relevance. Herein, we show that paraspeckles are assembled in luminal epithelial cells during mammary gland development. Importantly, genetic ablation of Neat1 results in aberrant mammary gland morphogenesis and lactation defects. We provide evidence that the lactation defect is caused by a decreased ability of Neat1-mutant cells to sustain high rates of proliferation during lobular-alveolar development. This study is the first to assign an important biological function to the lncRNA Neat1 and to link it to the presence of paraspeckles nuclear bodies in vivo.

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