A Rheological Study of Biodegradable Injectable PEGMC/HA Composite Scaffolds

Overview

Journal Soft Matter

Publisher Royal Society of Chemistry

Specialties Biochemistry
Chemistry

Date 2014 Oct 14

PMID 25309615

Citations 21

Authors

Yang Jiao

Dipendra Gyawali

Joseph M Stark

Pinar Akcora

Parvathi Nair

Richard T Tran

Jian Yang

Affiliations

Soon will be listed here.

Abstract

Injectable biodegradable hydrogels, which can be delivered in a minimally invasive manner and formed , have found a number of applications in pharmaceutical and biomedical applications, such as drug delivery and tissue engineering. We have recently developed an crosslinkable citric acid-based biodegradable poly (ethylene glycol) maleate citrate (PEGMC)/hydroxyapatite (HA) composite, which shows promise for use in bone tissue engineering. In this study, the mechanical properties of the PEGMC/HA composites were studied in dynamic linear rheology experiments. Critical parameters such as monomer ratio, crosslinker, initiator, and HA concentrations were varied to reveal their effect on the extent of crosslinking as they control the mechanical properties of the resultant gels. The rheological studies, for the first time, allowed us investigating the physical interactions between HA and citric acid-based PEGMC. Understanding the viscoelastic properties of the injectable gel composites is crucial in formulating suitable injectable PEGMC/HA scaffolds for bone tissue engineering, and should also promote the other biomedical applications based on citric acid-based biodegradable polymers.

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References

Habraken W, Wolke J, Jansen J . Ceramic composites as matrices and scaffolds for drug delivery in tissue engineering. Adv Drug Deliv Rev. 2007; 59(4-5):234-48. DOI: 10.1016/j.addr.2007.03.011. View

Chang C, Liao T, Hsu Y, Fang H, Chen C, Lin F . A poly(propylene fumarate)--calcium phosphate based angiogenic injectable bone cement for femoral head osteonecrosis. Biomaterials. 2010; 31(14):4048-55. DOI: 10.1016/j.biomaterials.2010.01.124. View

Gyawali D, Nair P, Zhang Y, Tran R, Zhang C, Samchukov M . Citric acid-derived in situ crosslinkable biodegradable polymers for cell delivery. Biomaterials. 2010; 31(34):9092-105. PMC: 2954112. DOI: 10.1016/j.biomaterials.2010.08.022. View

Dey J, Xu H, Shen J, Thevenot P, Gondi S, Nguyen K . Development of biodegradable crosslinked urethane-doped polyester elastomers. Biomaterials. 2008; 29(35):4637-49. PMC: 2747515. DOI: 10.1016/j.biomaterials.2008.08.020. View

Moursi A, Winnard A, Winnard P, Lannutti J, Seghi R . Enhanced osteoblast response to a polymethylmethacrylate-hydroxyapatite composite. Biomaterials. 2002; 23(1):133-44. DOI: 10.1016/s0142-9612(01)00088-6. View

Peter S, Kim P, Yasko A, Yaszemski M, Mikos A . Crosslinking characteristics of an injectable poly(propylene fumarate)/beta-tricalcium phosphate paste and mechanical properties of the crosslinked composite for use as a biodegradable bone cement. J Biomed Mater Res. 1999; 44(3):314-21. DOI: 10.1002/(sici)1097-4636(19990305)44:3<314::aid-jbm10>3.0.co;2-w. View

Tran R, Thevenot P, Zhang Y, Gyawali D, Tang L, Yang J . Scaffold Sheet Design Strategy for Soft Tissue Engineering. Nat Mater. 2010; 3(2):1375-1389. PMC: 2992388. DOI: 10.3390/ma3021375. View

Shi X, Hudson J, Spicer P, Tour J, Krishnamoorti R, Mikos A . Injectable nanocomposites of single-walled carbon nanotubes and biodegradable polymers for bone tissue engineering. Biomacromolecules. 2006; 7(7):2237-42. DOI: 10.1021/bm060391v. View

Khanna R, Katti K, Katti D . Bone nodules on chitosan-polygalacturonic acid-hydroxyapatite nanocomposite films mimic hierarchy of natural bone. Acta Biomater. 2010; 7(3):1173-83. DOI: 10.1016/j.actbio.2010.10.028. View

10.

Moura M, Figueiredo M, Gil M . Rheological study of genipin cross-linked chitosan hydrogels. Biomacromolecules. 2007; 8(12):3823-9. DOI: 10.1021/bm700762w. View

11.

Weng L, Chen X, Chen W . Rheological characterization of in situ crosslinkable hydrogels formulated from oxidized dextran and N-carboxyethyl chitosan. Biomacromolecules. 2007; 8(4):1109-15. PMC: 2572577. DOI: 10.1021/bm0610065. View

12.

Gyawali D, Tran R, Guleserian K, Tang L, Yang J . Citric-acid-derived photo-cross-linked biodegradable elastomers. J Biomater Sci Polym Ed. 2010; 21(13):1761-82. PMC: 2943534. DOI: 10.1163/092050609X12567178204169. View

13.

Peter S, Lu L, Kim D, Mikos A . Marrow stromal osteoblast function on a poly(propylene fumarate)/beta-tricalcium phosphate biodegradable orthopaedic composite. Biomaterials. 2000; 21(12):1207-13. DOI: 10.1016/s0142-9612(99)00254-9. View

14.

Ghosh K, Shu X, Mou R, Lombardi J, Prestwich G, Rafailovich M . Rheological characterization of in situ cross-linkable hyaluronan hydrogels. Biomacromolecules. 2005; 6(5):2857-65. DOI: 10.1021/bm050361c. View

15.

Yang J, Webb A, Pickerill S, Hageman G, Ameer G . Synthesis and evaluation of poly(diol citrate) biodegradable elastomers. Biomaterials. 2005; 27(9):1889-98. DOI: 10.1016/j.biomaterials.2005.05.106. View

16.

Yoshikawa T, Ohgushi H, Tamai S . Immediate bone forming capability of prefabricated osteogenic hydroxyapatite. J Biomed Mater Res. 1996; 32(3):481-92. DOI: 10.1002/(SICI)1097-4636(199611)32:3<481::AID-JBM23>3.0.CO;2-I. View

17.

Hu Y, Rawal A, Schmidt-Rohr K . Strongly bound citrate stabilizes the apatite nanocrystals in bone. Proc Natl Acad Sci U S A. 2010; 107(52):22425-9. PMC: 3012505. DOI: 10.1073/pnas.1009219107. View

18.

Lee K, Wang S, Yaszemski M, Lu L . Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites. Biomaterials. 2008; 29(19):2839-48. PMC: 2430424. DOI: 10.1016/j.biomaterials.2008.03.030. View

19.

Qiu H, Yang J, Kodali P, Koh J, Ameer G . A citric acid-based hydroxyapatite composite for orthopedic implants. Biomaterials. 2006; 27(34):5845-54. DOI: 10.1016/j.biomaterials.2006.07.042. View

20.

Tran R, Thevenot P, Gyawali D, Chiao J, Tang L, Yang J . Synthesis and characterization of a biodegradable elastomer featuring a dual crosslinking mechanism. Soft Matter. 2011; 6(11):2449-2461. PMC: 3233194. DOI: 10.1039/C001605E. View