Murine Natural Killer Cells Express Functional Fc Gamma Receptor II Encoded by the Fc Gamma R Alpha Gene

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1989 Jul 1

PMID 2526196

Citations 16

Authors

B Perussia

M M Tutt

W Q Qiu

W A Kuziel

P W Tucker

G Trinchieri

M Bennett

J V Ravetch

V Kumar

Affiliations

Soon will be listed here.

Abstract

We report evidence that murine NK cells express a functional Fc gamma RII encoded by the Fc gamma RII alpha gene. Several lines of indirect evidence indicate that freshly obtained NK cells from mice of several strains bear a functional Fc gamma RII: (a) anti-Fc gamma RII antibody 2.4G2 detects a small but significant proportion of sIg- cells and a small proportion of the 2.4G2+ cells are included in the Thy-1+ population; (b) sIg- lymphocytes contain 2.4G2+ and Fc gamma R-bearing cells in similar proportions; (c) binding of particulate immune complexes by sIg- lymphocytes is completely inhibited by 2.4G2; (d) 2.4G2+ cells mediate greater than 50% of the spontaneous cytotoxicity in sIg- splenic lymphocytes. Direct evidence for the presence of Fc gamma RII on murine NK cells is provided by the results of two-color immunofluorescence studies performed on splenic lymphocytes from C57BL/6 mice showing coexpression of NK-1.1 and 2.4G2. Studies of in vitro propagated homogeneous NK cell populations confirm that murine NK cells express only Fc gamma RII and that this Fc gamma R is functional, as shown in experiments of inhibition of ADCC by the anti-Fc gamma RII antibody 2.4G2. The results of studies at the molecular level show that an Fc gamma RII alpha transcript identical to that expressed in macrophages is the only molecule encoding Fc gamma RII in murine NK cells.

Citing Articles

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Fc receptors as adaptive immunoreceptors.

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NK cell self tolerance, responsiveness and missing self recognition.

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Nonstochastic coexpression of activation receptors on murine natural killer cells.

Smith H, Chuang H, Wang L, Salcedo M, Heusel J, Yokoyama W J Exp Med. 2000; 191(8):1341-54.

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Acquisition of Ly49 receptor expression by developing natural killer cells.

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