Electrostatic Interactions and Hydrogen Bond Dynamics in Chloride Pumping by Halorhodopsin

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 2014 Sep 27

PMID 25256652

Citations 2

Authors

Eduardo Jardon-Valadez

Ana-Nicoleta Bondar

Douglas J Tobias

Affiliations

Soon will be listed here.

Abstract

Translocation of negatively charged ions across cell membranes by ion pumps raises the question as to how protein interactions control the location and dynamics of the ion. Here we address this question by performing extensive molecular dynamics simulations of wild type and mutant halorhodopsin, a seven-helical transmembrane protein that translocates chloride ions upon light absorption. We find that inter-helical hydrogen bonds mediated by a key arginine group largely govern the dynamics of the protein and water groups coordinating the chloride ion.

Citing Articles

Physicochemical Evaluation of Micellar Solution and Lyotropic Phases Formed by Self-Assembled Aggregates of Morpholinium Geminis.

Bhadani A, Kafle A, Koura S, Sakai K, Sakai H, Abe M ACS Omega. 2019; 2(9):5324-5334.

PMID: 31457801 PMC: 6644452. DOI: 10.1021/acsomega.7b00428.

A Unique Light-Driven Proton Transportation Signal in Halorhodopsin from Natronomonas pharaonis.

Chen X, Huang Y, Yi H, Yang C Biophys J. 2016; 111(12):2600-2607.

PMID: 28002736 PMC: 5192691. DOI: 10.1016/j.bpj.2016.11.003.

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