A Biochemical Approach to Identify Direct MicroRNA Targets

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2014 Sep 22

PMID 25240884

Citations 19

Authors

Murugan Subramanian

Xiao Ling Li

Toshifumi Hara

Ashish Lal

Affiliations

Soon will be listed here.

Abstract

We have recently developed a biochemical approach to isolate miRNA-bound mRNAs and have used this method to identify the genome-wide mRNAs regulated by the tumor suppressor miRNA miR-34a. This method involves transfection of cells with biotinylated miRNA mimics, streptavidin pulldown, RNA isolation, and qRT-PCR. The protocol in this chapter describes these steps and the issues that should be considered while designing such pulldown experiments.

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