Analysis of Differential MiRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2014 Aug 22

PMID 25143946

Citations 32

Authors

Giuseppina Della Vittoria Scarpati

Enrica Calura

Mariacristina Di Marino

Chiara Romualdi

Luca Beltrame

Umberto Malapelle

Giancarlo Troncone

Alfonso De Stefano

Stefano Pepe

Sabino De Placido

Maurizio DIncalci

Sergio Marchini

Chiara Carlomagno

Affiliations

Soon will be listed here.

Abstract

Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.

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Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3.

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