High-resolution Mapping of Transcriptional Dynamics Across Tissue Development Reveals a Stable MRNA-tRNA Interface

Overview

Journal Genome Res

Specialty Genetics

Date 2014 Aug 15

PMID 25122613

Citations 56

Authors

Bianca M Schmitt

Konrad L M Rudolph

Panagiota Karagianni

Nuno A Fonseca

Robert J White

Iannis Talianidis

Duncan T Odom

John C Marioni

Claudia Kutter

Affiliations

Soon will be listed here.

Abstract

The genetic code is an abstraction of how mRNA codons and tRNA anticodons molecularly interact during protein synthesis; the stability and regulation of this interaction remains largely unexplored. Here, we characterized the expression of mRNA and tRNA genes quantitatively at multiple time points in two developing mouse tissues. We discovered that mRNA codon pools are highly stable over development and simply reflect the genomic background; in contrast, precise regulation of tRNA gene families is required to create the corresponding tRNA transcriptomes. The dynamic regulation of tRNA genes during development is controlled in order to generate an anticodon pool that closely corresponds to messenger RNAs. Thus, across development, the pools of mRNA codons and tRNA anticodons are invariant and highly correlated, revealing a stable molecular interaction interlocking transcription and translation.

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