Photoacoustic Microscopy and Computed Tomography: from Bench to Bedside

Overview

Journal Annu Rev Biomed Eng

Publisher Annual Reviews

Specialty Biomedical Engineering

Date 2014 Jun 7

PMID 24905877

Citations 80

Authors

Lihong V Wang

Liang Gao

Affiliations

Soon will be listed here.

Abstract

Photoacoustic imaging (PAI) of biological tissue has seen immense growth in the past decade, providing unprecedented spatial resolution and functional information at depths in the optical diffusive regime. PAI uniquely combines the advantages of optical excitation and those of acoustic detection. The hybrid imaging modality features high sensitivity to optical absorption and wide scalability of spatial resolution with the desired imaging depth. Here we first summarize the fundamental principles underpinning the technology, then highlight its practical implementation, and finally discuss recent advances toward clinical translation.

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References

Wei C, Xia J, Pelivanov I, Hu X, Gao X, ODonnell M . Trapping and dynamic manipulation of polystyrene beads mimicking circulating tumor cells using targeted magnetic/photoacoustic contrast agents. J Biomed Opt. 2012; 17(10):101517. PMC: 3418505. DOI: 10.1117/1.JBO.17.10.101517. View

Zhang C, Maslov K, Wang L . Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo. Opt Lett. 2010; 35(19):3195-7. PMC: 2952183. DOI: 10.1364/OL.35.003195. View

Maslov K, Zhang H, Hu S, Wang L . Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries. Opt Lett. 2008; 33(9):929-31. DOI: 10.1364/ol.33.000929. View

Culver J, Ntziachristos V, Holboke M, Yodh A . Optimization of optode arrangements for diffuse optical tomography: A singular-value analysis. Opt Lett. 2007; 26(10):701-3. DOI: 10.1364/ol.26.000701. View

Gao L, Zhang C, Li C, Wang L . Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry. Appl Phys Lett. 2013; 102(19):193705. PMC: 3669113. DOI: 10.1063/1.4807140. View

Jose J, Manohar S, Kolkman R, Steenbergen W, van Leeuwen T . Imaging of tumor vasculature using Twente photoacoustic systems. J Biophotonics. 2009; 2(12):701-17. DOI: 10.1002/jbio.200910025. View

Zhang E, Laufer J, Beard P . Backward-mode multiwavelength photoacoustic scanner using a planar Fabry-Perot polymer film ultrasound sensor for high-resolution three-dimensional imaging of biological tissues. Appl Opt. 2008; 47(4):561-77. DOI: 10.1364/ao.47.000561. View

Galanzha E, Shashkov E, Spring P, Suen J, Zharov V . In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser. Cancer Res. 2009; 69(20):7926-34. PMC: 2828368. DOI: 10.1158/0008-5472.CAN-08-4900. View

Telenkov S, Mandelis A . Photothermoacoustic imaging of biological tissues: maximum depth characterization comparison of time and frequency-domain measurements. J Biomed Opt. 2009; 14(4):044025. DOI: 10.1117/1.3200924. View

10.

Maslov K, Wang L . Photoacoustic imaging of biological tissue with intensity-modulated continuous-wave laser. J Biomed Opt. 2008; 13(2):024006. DOI: 10.1117/1.2904965. View

11.

Luke G, Yeager D, Emelianov S . Biomedical applications of photoacoustic imaging with exogenous contrast agents. Ann Biomed Eng. 2011; 40(2):422-37. DOI: 10.1007/s10439-011-0449-4. View

12.

Maugh 2nd T . Photoacoustic spectroscopy: new uses for an old technique. Science. 1975; 188(4183):38-9. DOI: 10.1126/science.188.4183.38. View

13.

Fang H, Maslov K, Wang L . Photoacoustic Doppler effect from flowing small light-absorbing particles. Phys Rev Lett. 2007; 99(18):184501. DOI: 10.1103/PhysRevLett.99.184501. View

14.

Wang Y, Wang L . Förster resonance energy transfer photoacoustic microscopy. J Biomed Opt. 2012; 17(8):086007. PMC: 3414237. DOI: 10.1117/1.JBO.17.8.086007. View

15.

Razansky D, Buehler A, Ntziachristos V . Volumetric real-time multispectral optoacoustic tomography of biomarkers. Nat Protoc. 2011; 6(8):1121-9. DOI: 10.1038/nprot.2011.351. View

16.

Zhang H, Maslov K, Stoica G, Wang L . Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging. Nat Biotechnol. 2006; 24(7):848-51. DOI: 10.1038/nbt1220. View

17.

Yao D, Maslov K, Shung K, Zhou Q, Wang L . In vivo label-free photoacoustic microscopy of cell nuclei by excitation of DNA and RNA. Opt Lett. 2010; 35(24):4139-41. PMC: 3048585. DOI: 10.1364/OL.35.004139. View

18.

Kim C, Erpelding T, Jankovic L, Wang L . Performance benchmarks of an array-based hand-held photoacoustic probe adapted from a clinical ultrasound system for non-invasive sentinel lymph node imaging. Philos Trans A Math Phys Eng Sci. 2011; 369(1955):4644-50. PMC: 3263783. DOI: 10.1098/rsta.2010.0353. View

19.

Nuster R, Schmitner N, Wurzinger G, Gratt S, Salvenmoser W, Meyer D . Hybrid photoacoustic and ultrasound section imaging with optical ultrasound detection. J Biophotonics. 2013; 6(6-7):549-59. DOI: 10.1002/jbio.201200223. View

20.

Maslov K, Stoica G, Wang L . In vivo dark-field reflection-mode photoacoustic microscopy. Opt Lett. 2005; 30(6):625-7. DOI: 10.1364/ol.30.000625. View