Identification of α-fodrin As an Autoantigen in Experimental Coronavirus Retinopathy (ECOR)

Overview

Journal J Neuroimmunol

Specialty Neurology

Date 2014 May 28

PMID 24864013

Citations 10

Authors

Marian S Chin

Laura C Hooper

John J Hooks

Barbara Detrick

Affiliations

Soon will be listed here.

Abstract

The coronavirus, mouse hepatitis virus (MHV), JHM strain induces a biphasic disease in BALB/c mice that consists of an acute retinitis followed by progression to a chronic retinal degeneration with autoimmune reactivity. Retinal degeneration resistant CD-1 mice do not develop either the late phase or autoimmune reactivity. A mouse RPE/choroid DNA expression library was screened using sera from virus infected BALB/c mice. Two clones were identified, villin-2 protein and α-fodrin protein. α-Fodrin protein was used for further analysis and western blot reactivity was seen only in sera from virus infected BALB/c mice. CD4 T cells were shown to specifically react with MHV antigens and with α-fodrin protein. These studies clearly identified both antibody and CD4 T cell reactivities to α-fodrin in sera from virus infected, retinal degenerative susceptible BALB/c mice.

Citing Articles

Ocular neuroinflammatory response secondary to SARS-CoV-2 infection-a review.

Zhao Y, Tang Y, Wang Q, Li J Front Immunol. 2025; 16:1515768.

PMID: 39967658 PMC: 11832381. DOI: 10.3389/fimmu.2025.1515768.

Retinal hemorrhage of late post-COVID-19 and post-vaccine-related pathogenic mechanisms: A new challenge for ophthalmologist in COVID era.

Amin M, Nahin S, Dola T, Afrin S, Hawlader M Clin Case Rep. 2022; 10(2):e05471.

PMID: 35223021 PMC: 8850941. DOI: 10.1002/ccr3.5471.

The Eye of the Storm: COVID-19 Vaccination and the Eye.

Ng X, Betzler B, Ng S, Chee S, Rajamani L, Singhal A Ophthalmol Ther. 2021; 11(1):81-100.

PMID: 34914035 PMC: 8675299. DOI: 10.1007/s40123-021-00415-5.

Combined endophthalmitis and orbital cellulitis in patients with corona virus disease (COVID-19).

Abdelkader M, Elshafei A, Nassar M, Abu Elela M, Abdallah R J Ophthalmic Inflamm Infect. 2021; 11(1):27.

PMID: 34523045 PMC: 8440116. DOI: 10.1186/s12348-021-00258-y.

Case Report: A Re-Positive Case of SARS-CoV-2 Associated With Glaucoma.

Zhou X, Zhou Y, Ali A, Liang C, Ye Z, Chen X Front Immunol. 2021; 12:701295.

PMID: 34394095 PMC: 8355891. DOI: 10.3389/fimmu.2021.701295.

References

Wang Y, Burnier M, Detrick B, Hooks J . Genetic predisposition to coronavirus-induced retinal disease. Invest Ophthalmol Vis Sci. 1996; 37(1):250-4. View

Thirkill C, Tait R, Tyler N, Roth A, Keltner J . The cancer-associated retinopathy antigen is a recoverin-like protein. Invest Ophthalmol Vis Sci. 1992; 33(10):2768-72. View

Heckenlively J, Fawzi A, Oversier J, Jordan B, Aptsiauri N . Autoimmune retinopathy: patients with antirecoverin immunoreactivity and panretinal degeneration. Arch Ophthalmol. 2000; 118(11):1525-33. DOI: 10.1001/archopht.118.11.1525. View

De Seze J, Dubucquoi S, Fauchais A, Matthias T, Devos D, Castelnovo G . Alpha-fodrin autoantibodies in the differential diagnosis of MS and Sjögren syndrome. Neurology. 2003; 61(2):268-9. DOI: 10.1212/01.wnl.0000071217.95981.0e. View

ROBBINS S, Detrick B, Hooks J . Retinopathy following intravitreal injection of mice with MHV strain JHM. Adv Exp Med Biol. 1990; 276:519-24. DOI: 10.1007/978-1-4684-5823-7_72. View

Adamus G, Machnicki M, Elerding H, Sugden B, Blocker Y, Fox D . Antibodies to recoverin induce apoptosis of photoreceptor and bipolar cells in vivo. J Autoimmun. 1998; 11(5):523-33. DOI: 10.1006/jaut.1998.0221. View

Bonilha V, Rayborn M, Saotome I, McClatchey A, Hollyfield J . Microvilli defects in retinas of ezrin knockout mice. Exp Eye Res. 2005; 82(4):720-9. DOI: 10.1016/j.exer.2005.09.013. View

ROBBINS S, Hamel C, Detrick B, Hooks J . Murine coronavirus induces an acute and long-lasting disease of the retina. Lab Invest. 1990; 62(4):417-26. View

Ren G, Adamus G . Cellular targets of anti-alpha-enolase autoantibodies of patients with autoimmune retinopathy. J Autoimmun. 2004; 23(2):161-7. DOI: 10.1016/j.jaut.2004.06.003. View

10.

Anderson M, Bluestone J . The NOD mouse: a model of immune dysregulation. Annu Rev Immunol. 2005; 23:447-85. DOI: 10.1146/annurev.immunol.23.021704.115643. View

11.

Rose N . The significance of autoimmunity in myocarditis. Ernst Schering Res Found Workshop. 2005; (55):141-54. DOI: 10.1007/3-540-30822-9_9. View

12.

Marina A, Cazorla P, Valdivieso F, Vazquez J . Anti-brain spectrin immunoreactivity in Alzheimer's disease: degradation of spectrin in an animal model of cholinergic degeneration. J Neuroimmunol. 1997; 77(1):91-8. DOI: 10.1016/s0165-5728(97)00066-0. View

13.

Detrick B, Lee M, Chin M, Hooper L, Chan C, Hooks J . Experimental coronavirus retinopathy (ECOR): retinal degeneration susceptible mice have an augmented interferon and chemokine (CXCL9, CXCL10) response early after virus infection. J Neuroimmunol. 2007; 193(1-2):28-37. PMC: 2562577. DOI: 10.1016/j.jneuroim.2007.09.032. View

14.

Redmond T, Yu S, Lee E, Bok D, HAMASAKI D, Chen N . Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle. Nat Genet. 1998; 20(4):344-51. DOI: 10.1038/3813. View

15.

Heckenlively J, Jordan B, Aptsiauri N . Association of antiretinal antibodies and cystoid macular edema in patients with retinitis pigmentosa. Am J Ophthalmol. 1999; 127(5):565-73. DOI: 10.1016/s0002-9394(98)00446-2. View

16.

Berryman M, Franck Z, BRETSCHER A . Ezrin is concentrated in the apical microvilli of a wide variety of epithelial cells whereas moesin is found primarily in endothelial cells. J Cell Sci. 1993; 105 ( Pt 4):1025-43. DOI: 10.1242/jcs.105.4.1025. View

17.

Kikuchi T, Arai J, Shibuki H, Kawashima H, Yoshimura N . Tubby-like protein 1 as an autoantigen in cancer-associated retinopathy. J Neuroimmunol. 2000; 103(1):26-33. DOI: 10.1016/s0165-5728(99)00163-0. View

18.

Janicke R, Ng P, Sprengart M, Porter A . Caspase-3 is required for alpha-fodrin cleavage but dispensable for cleavage of other death substrates in apoptosis. J Biol Chem. 1998; 273(25):15540-5. DOI: 10.1074/jbc.273.25.15540. View

19.

Isayama T, Goodman S, Zagon I . Spectrin isoforms in the mammalian retina. J Neurosci. 1991; 11(11):3531-8. PMC: 6575553. View

20.

Whitcup S, Vistica B, Milam A, Nussenblatt R, Gery I . Recoverin-associated retinopathy: a clinically and immunologically distinctive disease. Am J Ophthalmol. 1998; 126(2):230-7. DOI: 10.1016/s0002-9394(98)00149-4. View