Actin Scaffolding by Clathrin Heavy Chain is Required for Skeletal Muscle Sarcomere Organization

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2014 May 7

PMID 24798732

Citations 30

Authors

Stephane Vassilopoulos

Christel Gentil

Jeanne Laine

Pierre-Olivier Buclez

Agathe Franck

Arnaud Ferry

Guillaume Precigout

Robyn Roth

John E Heuser

Frances M Brodsky

Luis Garcia

Gisele Bonne

Thomas Voit

France Pietri-Rouxel

Marc Bitoun

Affiliations

Soon will be listed here.

Abstract

The ubiquitous clathrin heavy chain (CHC), the main component of clathrin-coated vesicles, is well characterized for its role in intracellular membrane traffic and endocytosis from the plasma membrane (PM). Here, we demonstrate that in skeletal muscle CHC regulates the formation and maintenance of PM-sarcomere attachment sites also known as costameres. We show that clathrin forms large coated lattices associated with actin filaments and the muscle-specific isoform of α-actinin at the PM of differentiated myotubes. Depletion of CHC in myotubes induced a loss of actin and α-actinin sarcomeric organization, whereas CHC depletion in vivo induced a loss of contractile force due to the detachment of sarcomeres from the PM. Our results suggest that CHC contributes to the formation and maintenance of the contractile apparatus through interactions with costameric proteins and highlight an unconventional role for CHC in skeletal muscle that may be relevant to pathophysiology of neuromuscular disorders.

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