Genomic Characterization of a Novel Group A Lamb Rotavirus Isolated in Zaragoza, Spain

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2008 Jul 29

PMID 18661221

Citations 12

Authors

Max Ciarlet

Christian Hoffmann

Eleonora Lorusso

Rafael Baselga

Maria Assunta Cafiero

Krisztian Banyai

Jelle Matthijnssens

Viviana Parreno

Simona De Grazia

Canio Buonavoglia

Vito Martella

Affiliations

Soon will be listed here.

Abstract

An ovine rotavirus (OVR) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in Zaragoza, Spain, and the VP4, VP7, VP6, NSP4, and NSP5/NSP6 genes were subsequently characterized molecularly. Strain OVR762 was classified as a P[14] rotavirus, as the VP4 and VP8* trypsin-cleavage product of the VP4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the P11[14] human rotavirus (HRV) strain PA169, isolated in Italy. Analysis of the VP7 gene product revealed that OVR762 possessed G8 serotype specificity, a type common in ruminants, with the highest degree of aa identity (95-98%) shared with serotype G8 HRV, bovine rotavirus, and guanaco (Lama guanicoe) rotavirus strains. Moreover, strain OVR762 displayed a bovine-like NSP4 (genotype E2) and NSP5/NSP6 (genotype H3), and a VP6 genotype I2, as well as a long electropherotype pattern. This is the first report of a lamb rotavirus with P[14] and G8 specificities, providing additional evidence for the wide genetic and antigenic diversity of group A rotaviruses.

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