Fluorescence Lifetime-based Contrast Enhancement of Indocyanine Green-labeled Tumors

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2017 Apr 12

PMID 28397959

Citations 10

Authors

Anand T N Kumar

Stefan A Carp

Jing Yang

Alana Ross

Zdravka Medarova

Chongzhao Ran

Affiliations

Soon will be listed here.

Abstract

Although the development of tumor-targeted fluorescent probes is a major area of investigation, it will be several years before these probes are realized for clinical use. Here, we report an approach that employs indocyanine-green (ICG), a clinically approved, nontargeted dye, in conjunction with fluorescence lifetime (FLT) detection to provide high accuracy for tumor-tissue identification in mouse models of subcutaneous human breast and brain tmors. The improved performance relies on the distinct FLTs of ICG within tumors versus tissue autofluorescence and is further aided by the well-known enhanced permeability and retention of ICG in tumors and the clearance of ICG from normal tissue several hours after intravenous injection. We demonstrate that FLT detection can provide more than 98% sensitivity and specificity, and a 10-fold reduction in error rates compared to intensity-based detection. Our studies suggest the significant potential of FLT-contrast for accurate tumor-tissue identification using ICG and other targeted probes under development, both for intraoperative imaging and for ex-vivo margin assessment of surgical specimens.

Citing Articles

High Spatiotemporal Near-Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor Margins.

Chen Z, Huang L, Gao D, Bao Z, Hu D, Zheng W Adv Sci (Weinh). 2024; 12(5):e2411272.

PMID: 39652447 PMC: 11791973. DOI: 10.1002/advs.202411272.

Fluorescence Imaging Using Deep-Red Indocyanine Blue, a Complementary Partner for Near-Infrared Indocyanine Green.

Gamage R, Smith B Chem Biomed Imaging. 2024; 2(5):384-397.

PMID: 38817322 PMC: 11134606. DOI: 10.1021/cbmi.4c00008.

Fluorescence imaging for real-time detection of breast cancer tumors using IV injection of indocyanine green with non-conventional imaging: a systematic review of preclinical and clinical studies of perioperative imaging technologies.

Pop C, Veys I, Bormans A, Larsimont D, Liberale G Breast Cancer Res Treat. 2024; 204(3):429-442.

PMID: 38182824 PMC: 10959791. DOI: 10.1007/s10549-023-07199-1.

Intraoperative molecular imaging: 3rd biennial clinical trials update.

Bou-Samra P, Muhammad N, Chang A, Karsalia R, Azari F, Kennedy G J Biomed Opt. 2023; 28(5):050901.

PMID: 37193364 PMC: 10182831. DOI: 10.1117/1.JBO.28.5.050901.

Design and characterization of a time-domain optical tomography platform for mesoscopic lifetime imaging.

Gao S, Li M, Smith J, Intes X Biomed Opt Express. 2022; 13(9):4637-4651.

PMID: 36187247 PMC: 9484415. DOI: 10.1364/BOE.460216.

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