Two-photon Excitation Improves Multifocal Structured Illumination Microscopy in Thick Scattering Tissue

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2014 Apr 8

PMID 24706872

Citations 35

Authors

Maria Ingaramo

Andrew G York

Peter Wawrzusin

Oleg Milberg

Amy Hong

Roberto Weigert

Hari Shroff

George H Patterson

Affiliations

Soon will be listed here.

Abstract

Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement beyond the diffraction limit at sample depths up to 50 µm, but scattered and out-of-focus light in thick samples degrades MSIM performance. Here we implement MSIM with a microlens array to enable efficient two-photon excitation. Two-photon MSIM gives resolution-doubled images with better sectioning and contrast in thick scattering samples such as Caenorhabditis elegans embryos, Drosophila melanogaster larval salivary glands, and mouse liver tissue.

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