Crystallization and Preliminary X-ray Diffraction Analysis of the Interleukin-3 Alpha Receptor Bound to the Fab Fragment of Antibody CSL362

Overview

Journal Acta Crystallogr F Struct Biol Commun

Specialty Chemistry

Date 2014 Mar 7

PMID 24598927

Citations 4

Authors

Sophie E Broughton

Timothy R Hercus

Tracy L Nero

Urmi Dhagat

Catherine M Owczarek

Matthew P Hardy

Louis J Fabri

Pierre D Scotney

Andrew D Nash

Nicholas J Wilson

Angel F Lopez

Michael W Parker

Affiliations

Soon will be listed here.

Abstract

Interleukin-3 (IL-3) is a member of the beta common family of cytokines that regulate multiple functions of myeloid cells. The IL-3 receptor-specific alpha subunit (IL3Rα) is overexpressed on stem cells/progenitor cells of patients with acute myeloid leukaemia, where elevated receptor expression correlates clinically with a reduced patient survival rate. The monoclonal antibody (MAb) CSL362 is a humanized MAb derived from the murine MAb 7G3, originally identified for its ability to specifically recognize the human IL-3 receptor and for blocking the signalling of IL-3 in myeloid and endothelial cells. In order to elucidate the molecular mechanism of CSL362 antagonism, a preliminary structure of human IL3Rα in complex with the MAb CSL362 has been determined.

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