Engrailed-2 is Down-regulated but Also Ectopically Expressed in Clear Cell Renal Cell Carcinoma

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2014 Feb 18

PMID 24532139

Citations 8

Authors

Cai-Yong Lai

Bin Pan

Yun Luo

Wei-Bo Liang

Jie Chen

Dong-ming Ye

Ji-Nan Guo

Li Li

Ze-Xuan Su

Affiliations

Soon will be listed here.

Abstract

Engrailed-2 (EN2), a member of the homeobox family of genes, encodes a homeodomain-containing transcription factor that is thought to be a potential oncogene in a number of cancers. Because the role of EN2 in clear cell renal cell carcinoma (CCRCC) has not been determined, we investigated its expression in CCRCC tissues and cell lines. Using immunohistochemical (IHC) staining, we found that EN2 protein was expressed in normal renal cells and tubules, but was frequently down-regulated in tissues from patients with CCRCC and in CCRCC cell lines. In addition, we found that EN2, which functions in the nucleus, was completely localized to the cytoplasm of CCRCC cells as detected by IHC and immunofluorescence staining. Furthermore, expression of EN2 protein was negatively correlated with increasing histological grade of CCRCC tumors (P = 0.003). The exact role of EN2 expression in renal carcinoma carcinogenesis requires further investigation.

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