Upregulation of Centromere Protein H is Associated with Progression of Renal Cell Carcinoma

Overview

Journal J Mol Histol

Specialty Biochemistry

Date 2015 Aug 8

PMID 26248586

Citations 13

Authors

Xun Wu

Youcheng Lin

Liping Shi

Yi Huang

Caiyong Lai

Yongqiang Wang

Meng Zhang

Shupeng Wang

Baoli Heng

Ganshen Yu

Xinghua Du

Lu Fang

Yu Fu

Jie Chen

Zexiong Guo

Zexuan Su

Song Wu

Affiliations

Soon will be listed here.

Abstract

Centromere protein H (CENPH), one of the essential component of active kinetochore, plays an important role in carcinogenesis of many cancer types. However, its expression signature and prognostic significance of renal cell carcinoma (RCC) are unclear. In the present study, we concluded that the expression of CENPH was prominently upregulated in RCC specimens and three RCC cell lines (ACHN, 786-O and A704). Immunohistochemical analysis revealed that RCCs exhibited higher levels of CENPH expression than normal renal tissues in paraffin-embedded archival specimens. Further statistical analysis suggested the upregulation of CENPH was positively correlated with the Fuhrman grade (P = 0.001), distant metastasis (P = 0.024) and clinical stage (P = 0.014). In addition, the CENPH served as an independent predictor of overall survival of RCC patients in multivariate analysis (P = 0.018). Furthermore, our in vitro assays of RCC cell lines indicated that knockdown of CENPH reduced cell proliferation, inhibited cell growth, and increased cell apoptosis. In conclusion, our data suggest that CENPH is a novel molecule involved in RCC progression, which provides a potential biomarker and therapeutic target.

Citing Articles

CENP-H as a new prognostic biomarker for tumors: a real-world literature review.

Li Q, Tang Y, Zuo J, Han H, Tu G, Chen C Front Oncol. 2025; 15:1521988.

PMID: 40071086 PMC: 11893413. DOI: 10.3389/fonc.2025.1521988.

Comprehensive bioinformatics analysis of prognosis and immunotherapy in lung adenocarcinoma.

Peng L, Xia L, Yang M, Wen Y, Zeng Q J Thorac Dis. 2025; 16(12):8633-8647.

PMID: 39831256 PMC: 11740037. DOI: 10.21037/jtd-24-1530.

Development and validation of disulfidptosis-related genes signature for patients with glioma.

Wang J, Luo J, Yang S, Deng Y, Chen P, Tan Y Discov Oncol. 2024; 15(1):758.

PMID: 39692962 PMC: 11655816. DOI: 10.1007/s12672-024-01664-z.

TFRC-RNA interactions show the regulation of gene expression and alternative splicing associated with IgAN in human renal tubule mesangial cells.

Li J, Chen X, Luo A, Chen D Front Genet. 2023; 14:1176118.

PMID: 37547464 PMC: 10397801. DOI: 10.3389/fgene.2023.1176118.

Pan-Cancer Analysis Reveals CENPI as a Potential Biomarker and Therapeutic Target in Adrenocortical Carcinoma.

Wu F, Li G, Shen H, Huang J, Liu Z, Zhu Y J Inflamm Res. 2023; 16:2907-2928.

PMID: 37465344 PMC: 10350421. DOI: 10.2147/JIR.S408358.

References

Shigeishi H, Higashikawa K, Ono S, Mizuta K, Ninomiya Y, Yoneda S . Increased expression of CENP-H gene in human oral squamous cell carcinomas harboring high-proliferative activity. Oncol Rep. 2006; 16(5):1071-5. View

Ljungberg B, Bensalah K, Canfield S, Dabestani S, Hofmann F, Hora M . EAU guidelines on renal cell carcinoma: 2014 update. Eur Urol. 2015; 67(5):913-24. DOI: 10.1016/j.eururo.2015.01.005. View

Rajagopalan H, Lengauer C . Aneuploidy and cancer. Nature. 2004; 432(7015):338-41. DOI: 10.1038/nature03099. View

Volpe A, Jacques Patard J . Prognostic factors in renal cell carcinoma. World J Urol. 2010; 28(3):319-27. DOI: 10.1007/s00345-010-0540-8. View

Fukagawa T, Mikami Y, Nishihashi A, Regnier V, Haraguchi T, Hiraoka Y . CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells. EMBO J. 2001; 20(16):4603-17. PMC: 125570. DOI: 10.1093/emboj/20.16.4603. View

Zamparese R, Pannone G, Santoro A, Lo Muzio L, Corsi F, Pedicillo M . Survivin expression in renal cell carcinoma. Cancer Invest. 2008; 26(9):929-35. DOI: 10.1080/07357900802017553. View

Liao W, Song L, Zhang H, Zhang X, Zhang L, Liu W . Centromere protein H is a novel prognostic marker for nasopharyngeal carcinoma progression and overall patient survival. Clin Cancer Res. 2007; 13(2 Pt 1):508-14. DOI: 10.1158/1078-0432.CCR-06-1512. View

Guo X, Zhang G, Wang J, Liu W, Wang F, Dong J . Prognostic relevance of Centromere protein H expression in esophageal carcinoma. BMC Cancer. 2008; 8:233. PMC: 2535782. DOI: 10.1186/1471-2407-8-233. View

Ljungberg B . Prognostic markers in renal cell carcinoma. Curr Opin Urol. 2007; 17(5):303-8. DOI: 10.1097/MOU.0b013e328277f180. View

10.

Kramer A, Neben K, Ho A . Centrosome replication, genomic instability and cancer. Leukemia. 2002; 16(5):767-75. DOI: 10.1038/sj.leu.2402454. View

11.

Rini B, Campbell S, Escudier B . Renal cell carcinoma. Lancet. 2009; 373(9669):1119-32. DOI: 10.1016/S0140-6736(09)60229-4. View

12.

Nicholson J, Cimini D . Link between aneuploidy and chromosome instability. Int Rev Cell Mol Biol. 2015; 315:299-317. DOI: 10.1016/bs.ircmb.2014.11.002. View

13.

Liao W, Feng Y, Li M, Liu G, Li M, Zeng M . Overexpression of centromere protein H is significantly associated with breast cancer progression and overall patient survival. Chin J Cancer. 2011; 30(9):627-37. PMC: 4013325. DOI: 10.5732/cjc.010.10599. View

14.

Shi X, Jiang J, Ye X, Liu Y, Wu Q, Wang L . Prognostic prediction and diagnostic role of intercellular adhesion molecule-1 (ICAM1) expression in clear cell renal cell carcinoma. J Mol Histol. 2014; 45(4):427-34. DOI: 10.1007/s10735-014-9568-1. View

15.

Vincenzi B, Santini D, Tonini G . Renal-cell carcinoma. N Engl J Med. 2006; 354(10):1095-6. View

16.

Duensing A, Duensing S . Centrosomes, polyploidy and cancer. Adv Exp Med Biol. 2010; 676:93-103. DOI: 10.1007/978-1-4419-6199-0_6. View

17.

Sugata N, Munekata E, Todokoro K . Characterization of a novel kinetochore protein, CENP-H. J Biol Chem. 1999; 274(39):27343-6. DOI: 10.1074/jbc.274.39.27343. View

18.

Liao W, Wang X, Xu L, Kong Q, Yu C, Li M . Centromere protein H is a novel prognostic marker for human nonsmall cell lung cancer progression and overall patient survival. Cancer. 2009; 115(7):1507-17. DOI: 10.1002/cncr.24128. View

19.

Yu Z, Ni L, Chen D, Zhang Q, Su Z, Wang Y . Identification of miR-7 as an oncogene in renal cell carcinoma. J Mol Histol. 2013; 44(6):669-77. DOI: 10.1007/s10735-013-9516-5. View

20.

Krambeck A, Dong H, Thompson R, Kuntz S, Lohse C, Leibovich B . Survivin and b7-h1 are collaborative predictors of survival and represent potential therapeutic targets for patients with renal cell carcinoma. Clin Cancer Res. 2007; 13(6):1749-56. DOI: 10.1158/1078-0432.CCR-06-2129. View