Lipidic Cubic Phase Injector Facilitates Membrane Protein Serial Femtosecond Crystallography

Overview

Journal Nat Commun

Specialty Biology

Date 2014 Feb 15

PMID 24525480

Citations 277

Authors

Uwe Weierstall

Daniel James

Chong Wang

Thomas A White

Dingjie Wang

Wei Liu

John C H Spence

R Bruce Doak

Garrett Nelson

Petra Fromme

Raimund Fromme

Ingo Grotjohann

Christopher Kupitz

Nadia A Zatsepin

Haiguang Liu

Shibom Basu

Daniel Wacker

Gye Won Han

Vsevolod Katritch

Sebastien Boutet

Marc Messerschmidt

Garth J Williams

Jason E Koglin

M Marvin Seibert

Markus Klinker

Cornelius Gati

Robert L Shoeman

Anton Barty

Henry N Chapman

Richard A Kirian

Kenneth R Beyerlein

Raymond C Stevens

Dianfan Li

Syed T A Shah

Nicole Howe

Martin Caffrey

Vadim Cherezov

Affiliations

Soon will be listed here.

Abstract

Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

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