Polyamines in Biological Samples: Rapid and Robust Quantification by Solid-phase Extraction Online-coupled to Liquid Chromatography-tandem Mass Spectrometry

Overview

Journal J Chromatogr A

Publisher Elsevier

Specialty Chemistry

Date 2014 Feb 4

PMID 24485539

Citations 26

Authors

Christoph Magnes

Alexander Fauland

Edgar Gander

Sophie Narath

Maria Ratzer

Tobias Eisenberg

Frank Madeo

Thomas Pieber

Frank Sinner

Affiliations

Soon will be listed here.

Abstract

Polyamines are ubiquitous active biogenic amines which contribute to basic cellular functions. Hence, their quantification in samples of diverse biological origins is essential for understanding how they function, especially in disease-relevant conditions. We present here a robust, high-throughput solid-phase extraction online coupled to a liquid chromatography-tandem mass spectrometry (SPE-LC/MS/MS) approach for the simultaneous quantification of eight polyamines in various biological samples. The polyamines include 1,3-diaminopropane, putrescine, cadaverin, N-acetyl-putrescine, spermidine, spermine, N(1)-acetyl-spermine, and l-ornithine. The novelty of the work is the use of two SPE columns online coupled to LC/MS/MS, which minimizes the sample pretreatment to a single derivatization step. The analysis is complete within 4min, making the method highly suitable for routine clinical analysis and high throughput screenings. The method was fully validated with serum samples. Dynamic ranges were 0.03 to 15μg/ml for ornithine and 1 to 500ng/ml for other polyamines, which cover physiological concentrations in serum samples. Lower limits of quantification (LLoQ) were found to be between 0.1 and 5ng/ml. As a proof of concept, we investigated gender differences in polyamine levels by analyzing the serum levels of 102 subjects.

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