Spermidine or Spermine is Essential for the Aerobic Growth of Saccharomyces Cerevisiae

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1991 Jul 1

PMID 2062864

Citations 40

Authors

D Balasundaram

C W TABOR

H Tabor

Affiliations

Soon will be listed here.

Abstract

A null mutation in the SPE2 gene of Saccharomyces cerevisiae, encoding S-adenosylmethionine decarboxylase, results in cells with no detectable S-adenosylmethionine decarboxylase, spermidine, and spermine. This mutant has an absolute requirement for spermidine or spermine for growth; this requirement is not satisfied by putrescine. Polyamine-depleted cells show a number of microscopic abnormalities that are similar to those reported for several cell division cycle (cdc) and actin mutants. These include a striking increase in cell size, a marked decrease in budding, accumulation of vesicle-like bodies, absence of specific localization of chitin-like material, and abnormal distribution of actin-like material. The absolute requirement for polyamines for growth and the microscopic abnormalities are not seen if the cultures are grown under anaerobic conditions.

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