Adipose Tissue Angiogenesis Assay

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2014 Feb 1

PMID 24480342

Citations 18

Authors

Raziel Rojas-Rodriguez

Olga Gealekman

Maxwell E Kruse

Brittany Rosenthal

Kishore Rao

Soyun Min

Karl D Bellve

Lawrence M Lifshitz

Silvia Corvera

Affiliations

Soon will be listed here.

Abstract

Changes in adipose tissue mass must be accompanied by parallel changes in microcirculation. Investigating the mechanisms that regulate adipose tissue angiogenesis could lead to better understanding of adipose tissue function and reveal new potential therapeutic strategies. Angiogenesis is defined as the formation of new capillaries from existing microvessels. This process can be recapitulated in vitro, by incubation of tissue in extracellular matrix components in the presence of pro-angiogenic factors. Here, we describe a method to study angiogenesis from adipose tissue fragments obtained from mouse and human tissue. This assay can be used to define effects of diverse factors added in vitro, as well as the role of endogenously produced factors on angiogenesis. We also describe approaches to quantify angiogenic potential for the purpose of enabling comparisons between subjects, thus providing information on the role of physiological conditions of the donor on adipose tissue angiogenic potential.

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