Modulation of Carcinoembryonic Antigen Release by HT-29 Colon Carcinoma Line in the Presence of Different Agents

Overview

Journal Experientia

Specialty Science

Date 1987 Oct 15

PMID 2444456

Citations 4

Authors

J Friedman

M Seger

H Levinsky

D Allalouf

Affiliations

Soon will be listed here.

Abstract

In this study we followed the effects of various differentiating agents on the expression of carcinoembryonic antigen (CEA) released into the medium by a colon carcinoma cell line HT-29. Butyric acid 1 mM markedly increased the level of CEA (12-fold in comparison to control levels). 12-O-tetradecanoyl-phorbol-13-acetate (TPA) 50 ng/ml and 5-azacytidine 4 x 10(-6) M increased the amount of CEA, 2- and 1.5-fold respectively. On the other hand retinoic acid 10(-5) M, N methyl-formamide 1% and N,N hexamethylene bisacetamide 2.5 mM decreased CEA 2-, 4- and 3-fold respectively. Our results emphasize that various differentiating agents affect CEA levels differently. Thus changes in CEA levels appear not to be reliable as a marker of a more differentiated phenotype.

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References

Gusella J, Geller R, Clarke B, Weeks V, Housman D . Commitment to erythroid differentiation by friend erythroleukemia cells: a stochastic analysis. Cell. 1976; 9(2):221-9. DOI: 10.1016/0092-8674(76)90113-6. View

Taffet S, GREENFIELD A, Haddox M . Retinal inhibits TPA activated, calcium-dependent, phospholipid-dependent protein kinase ("C" kinase). Biochem Biophys Res Commun. 1983; 114(3):1194-9. DOI: 10.1016/0006-291x(83)90689-7. View

Collins S, Ruscetti F, Gallagher R, Gallo R . Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc Natl Acad Sci U S A. 1978; 75(5):2458-62. PMC: 392573. DOI: 10.1073/pnas.75.5.2458. View

Huberman E, Callaham M . Induction of terminal differentiation in human promyelocytic leukemia cells by tumor-promoting agents. Proc Natl Acad Sci U S A. 1979; 76(3):1293-7. PMC: 383237. DOI: 10.1073/pnas.76.3.1293. View

Leavitt J, Barrett J, Crawford B, Tso P . Butyric acid suppression of the in vitro neoplastic state of Syrian hamster cells. Nature. 1978; 271(5642):262-5. DOI: 10.1038/271262a0. View

Nordenberg J, Wasserman L, Beery E, Aloni D, Malik H, STENZEL K . Growth inhibition of murine melanoma by butyric acid and dimethylsulfoxide. Exp Cell Res. 1986; 162(1):77-85. DOI: 10.1016/0014-4827(86)90427-1. View

Rabson A, Stern R, Tralka T, Costa J, Wilczek J . Hexamethylene bisacetamide induces morphologic changes and increased synthesis of procollagen in cell line from glioblastoma multiforme. Proc Natl Acad Sci U S A. 1977; 74(11):5060-4. PMC: 432098. DOI: 10.1073/pnas.74.11.5060. View

Bauer G, Hofler P, zur Hausen H . Epstein-Barr virus induction by a serum factor. I. Induction and cooperation with additional inducers. Virology. 1982; 121(1):184-94. DOI: 10.1016/0042-6822(82)90128-3. View

Shi Z, Tsao D, Kim Y . Subcellular distribution, synthesis, and release of carcinoembryonic antigen in cultured human colon adenocarcinoma cell lines. Cancer Res. 1983; 43(9):4045-9. View

10.

Siddiqui B, Kim Y . Effects of sodium butyrate, dimethyl sulfoxide, and retinoic acid on glycolipids of human rectal adenocarcinoma cells. Cancer Res. 1984; 44(4):1648-52. View

11.

Goslin R, OBrien M, Steele G, Mayer R, Wilson R, Corson J . Correlation of Plasma CEA and CEA tissue staining in poorly differentiated colorectal cancer. Am J Med. 1981; 71(2):246-53. DOI: 10.1016/0002-9343(81)90125-x. View

12.

Colburn N, Ozanne S, Lichti U, Ben T, Yuspa S, Wendel E . Retinoids inhibit promoter-dependent preneoplastic progression in mouse epidermal cell lines. Ann N Y Acad Sci. 1981; 359:251-9. DOI: 10.1111/j.1749-6632.1981.tb12751.x. View

13.

Lotan R . Effects of vitamin A and its analogs (retinoids) on normal and neoplastic cells. Biochim Biophys Acta. 1980; 605(1):33-91. DOI: 10.1016/0304-419x(80)90021-9. View

14.

Collins S, Bodner A, Ting R, Gallo R . Induction of morphological and functional differentiation of human promyelocytic leukemia cells (HL-60) by componuds which induce differentiation of murine leukemia cells. Int J Cancer. 1980; 25(2):213-8. DOI: 10.1002/ijc.2910250208. View

15.

Kim Y, Tsao D, Siddiqui B, WHITEHEAD J, ARNSTEIN P, Bennett J . Effects of sodium butyrate and dimethylsulfoxide on biochemical properties of human colon cancer cells. Cancer. 1980; 45(5 Suppl):1185-92. DOI: 10.1002/1097-0142(19800315)45:5+<1185::aid-cncr2820451324>3.0.co;2-w. View

16.

Tsao D, Morita A, Bella Jr A, Luu P, Kim Y . Differential effects of sodium butyrate, dimethyl sulfoxide, and retinoic acid on membrane-associated antigen, enzymes, and glycoproteins of human rectal adenocarcinoma cells. Cancer Res. 1982; 42(3):1052-8. View

17.

Dexter D, Barbosa J, Calabresi P . N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res. 1979; 39(3):1020-5. View

18.

Augeron C, Laboisse C . Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Res. 1984; 44(9):3961-9. View

19.

Wagener C, Nisson S, Groner J, Breuer H . Localization and concentration of carcinoembryonic antigen (CEA) in gastrointestinal tumors: correlation with CEA levels in plasma. J Natl Cancer Inst. 1981; 67(3):539-47. View

20.

Riggs A, Jones P . 5-methylcytosine, gene regulation, and cancer. Adv Cancer Res. 1983; 40:1-30. DOI: 10.1016/s0065-230x(08)60678-8. View