Function of CSE1L/CAS in the Secretion of HT-29 Human Colorectal Cells and Its Expression in Human Colon

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2009 Feb 19

PMID 19224336

Citations 10

Authors

Tang-Yi Tsao

Chin-Shaw Stella Tsai

Jai-Nien Tung

Shun-Liang Chen

Chia-Herng Yue

Ching-Fong Liao

Chi-Chao Wang

Ming-Chung Jiang

Affiliations

Soon will be listed here.

Abstract

The secretion of colorectal epithelium is important for maintaining the physiological function of colorectal organ. Herein, we report that cellular apoptosis susceptibility (CAS) (or CSE1L) protein regulates the secretion of HT-29 human colorectal cells. Polarity is essential for directed secretion of substances produced by epithelial cells to the external (luminal) compartment; CAS overexpression induced polarization of HT-29 cells. CAS was punctate stained in the cytoplasm of HT-29 cells, and CAS overexpression increased the translocation of CAS-stained vesicles to the cytoplasm near cell membrane and cell protrusions. CAS overexpression increased the secretion of carcinoembryonic antigen (CEA) and cathepsin D. Immunohistochemistry showed CAS was positively stained in the goblet cells of colon mucosa and cells in the crypts of Lieberkühn of human colon as well as the glands in metastatic colorectal cancer tissue. Our results suggest that CAS regulates the secretion of colorectal cells and may regulate the metastasis of colorectal cancer.

Citing Articles

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