Crystallization and Preliminary X-ray Diffraction Studies of the (R)-selective Amine Transaminase from Aspergillus Fumigatus

Overview

Journal Acta Crystallogr Sect F Struct Biol Cryst Commun

Date 2013 Dec 10

PMID 24316843

Citations 3

Authors

Maren Thomsen

Lilly Skalden

Gottfried J Palm

Matthias Hohne

Uwe T Bornscheuer

Winfried Hinrichs

Affiliations

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Abstract

The (R)-selective amine transaminase from Aspergillus fumigatus was expressed in Escherichia coli and purified to homogeneity. Bright yellow crystals appeared while storing the concentrated solution in the refrigerator and belonged to space group C222(1). X-ray diffraction data were collected to 1.27 Å resolution, as well as an anomalous data set to 1.84 Å resolution that was suitable for S-SAD phasing.

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