Protocol to Isolate a Large Amount of Functional Oligodendrocyte Precursor Cells from the Cerebral Cortex of Adult Mice and Humans

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Dec 5

PMID 24303061

Citations 17

Authors

Eva Maria Medina-Rodriguez

Francisco Javier Arenzana

Ana Bribian

Fernando de Castro

Affiliations

Soon will be listed here.

Abstract

During development, oligodendrocytes are generated from oligodendrocyte precursor cells (OPCs), a cell type that is a significant proportion of the total cells (3-8%) in the adult central nervous system (CNS) of both rodents and humans. Adult OPCs are responsible for the spontaneous remyelination that occurs in demyelinating diseases like Multiple Sclerosis (MS) and they constitute an interesting source of cells for regenerative therapy in such conditions. However, there is little data regarding the neurobiology of adult OPCs isolated from mice since an efficient method to isolate them has yet to be established. We have designed a protocol to obtain viable adult OPCs from the cerebral cortex of different mouse strains and we have compared its efficiency with other well-known methods. In addition, we show that this protocol is also useful to isolate functional OPCs from human brain biopsies. Using this method we can isolate primary cortical OPCs in sufficient quantities so as to be able to study their survival, maturation and function, and to facilitate an evaluation of their utility in myelin repair.

Citing Articles

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Gomez-Martin P, Macias-Castellano A, Fernandez-Gomez B, Sanchez-Jimenez E, Marchena M, de Castro F Methods Mol Biol. 2025; 2899:47-57.

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The Journey of iPSC-derived OPCs in Demyelinating Disorders: From Generation to Transplantation.

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Malara M, Lutz A, Incearap B, Bauer H, Cursano S, Volbracht K Cell Mol Life Sci. 2022; 79(7):371.

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