Transcription Factor-mediated Reprogramming of Fibroblasts to Expandable, Myelinogenic Oligodendrocyte Progenitor Cells

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2013 Apr 16

PMID 23584611

Citations 142

Authors

Fadi J Najm

Angela M Lager

Anita Zaremba

Krysta Wyatt

Andrew V Caprariello

Daniel C Factor

Robert T Karl

Tadao Maeda

Robert H Miller

Paul J Tesar

Affiliations

Soon will be listed here.

Abstract

Cell-based therapies for myelin disorders, such as multiple sclerosis and leukodystrophies, require technologies to generate functional oligodendrocyte progenitor cells. Here we describe direct conversion of mouse embryonic and lung fibroblasts to induced oligodendrocyte progenitor cells (iOPCs) using sets of either eight or three defined transcription factors. iOPCs exhibit a bipolar morphology and global gene expression profile consistent with bona fide OPCs. They can be expanded in vitro for at least five passages while retaining the ability to differentiate into multiprocessed oligodendrocytes. When transplanted to hypomyelinated mice, iOPCs are capable of ensheathing host axons and generating compact myelin. Lineage conversion of somatic cells to expandable iOPCs provides a strategy to study the molecular control of oligodendrocyte lineage identity and may facilitate neurological disease modeling and autologous remyelinating therapies.

Citing Articles

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