MicroRNA-33 Regulates Sterol Regulatory Element-binding Protein 1 Expression in Mice

Overview

Journal Nat Commun

Specialty Biology

Date 2013 Dec 5

PMID 24300912

Citations 110

Authors

Takahiro Horie

Tomohiro Nishino

Osamu Baba

Yasuhide Kuwabara

Tetsushi Nakao

Masataka Nishiga

Shunsuke Usami

Masayasu Izuhara

Naoya Sowa

Naoya Yahagi

Hitoshi Shimano

Shigenobu Matsumura

Kazuo Inoue

Hiroyuki Marusawa

Tomoyuki Nakamura

Koji Hasegawa

Noriaki Kume

Masayuki Yokode

Toru Kita

Takeshi Kimura

Koh Ono

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRs) are small non-protein-coding RNAs that bind to specific mRNAs and inhibit translation or promote mRNA degradation. Recent reports have indicated that miR-33, which is located within the intron of sterol regulatory element-binding protein (SREBP) 2, controls cholesterol homoeostasis and may be a potential therapeutic target for the treatment of atherosclerosis. Here we show that deletion of miR-33 results in marked worsening of high-fat diet-induced obesity and liver steatosis. Using miR-33(-/-)Srebf1(+/-) mice, we demonstrate that SREBP-1 is a target of miR-33 and that the mechanisms leading to obesity and liver steatosis in miR-33(-/-) mice involve enhanced expression of SREBP-1. These results elucidate a novel interaction between SREBP-1 and SREBP-2 mediated by miR-33 in vivo.

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