Isolation and Characterization of CDNA Clones Encoding the 17.9 and 8.1 KDa Subunits of Photosystem I from Chlamydomonas Reinhardtii

Overview

Journal Plant Mol Biol

Date 2013 Nov 26

PMID 24272906

Citations 14

Authors

L G Franzen

G FRANK

H Zuber

J D Rochaix

Affiliations

Soon will be listed here.

Abstract

cDNA clones encoding two Photosystem I subunits of Chlamydomonas reinhardtii with apparent molecular masses of 18 and 11 kDa (thylakoid polypeptides 21 and 30; P21 and P30 respectively) were isolated using oligonucleotides, the sequences of which were deduced from the N-terminal amino acid sequences of the proteins. The cDNAs were sequenced and used to probe Southern and Northern blots. The Southern blot analysis indicates that both proteins are encoded by single-copy genes. The mRNA sizes of the two components are 1400 and 740 nucleotides, respectively. Comparison between the open reading frames of the cDNAs and the N-terminal amino acid sequences of the proteins indicates that the molecular masses of the mature proteins are 17.9 (P21) and 8.1 kDa (P30). Analysis of the deduced protein sequences predicts that both subunits are extrinsic membrane proteins with net positive charges. The amino acid sequences of the transit peptides suggest that P21 and P30 are routed towards the lumenal and stromal sides of the thylakoid membranes, respectively.

Citing Articles

Anomalous electron transport activity in a Photosystem I-deficient maize mutant.

Cook W, Miles D Photosynth Res. 2014; 24(1):81-8.

PMID: 24419768 DOI: 10.1007/BF00032647.

Extrinsic polypeptides of spinach photosystem I.

Tjus S, Andersson B Photosynth Res. 2014; 27(3):209-19.

PMID: 24414693 DOI: 10.1007/BF00035842.

Changes in composition of membrane proteins accompanying the regulation of PS I/PS II stoichiometry observed with Synechocystis PCC 6803.

Aizawa K, Shimizu T, Hiyama T, Satoh K, Nakamura Y Photosynth Res. 2014; 32(2):131-8.

PMID: 24408283 DOI: 10.1007/BF00035947.

Biochemical and structural studies of the large Ycf4-photosystem I assembly complex of the green alga Chlamydomonas reinhardtii.

Ozawa S, Nield J, Terao A, Stauber E, Hippler M, Koike H Plant Cell. 2009; 21(8):2424-42.

PMID: 19700633 PMC: 2751955. DOI: 10.1105/tpc.108.063313.

The N-terminal domain of PsaF: precise recognition site for binding and fast electron transfer from cytochrome c6 and plastocyanin to photosystem I of Chlamydomonas reinhardtii.

Hippler M, Drepper F, Haehnel W, Rochaix J Proc Natl Acad Sci U S A. 1998; 95(13):7339-44.

PMID: 9636150 PMC: 22610. DOI: 10.1073/pnas.95.13.7339.

References

Goldschmidt-Clermont M, Rahire M . Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. J Mol Biol. 1986; 191(3):421-32. DOI: 10.1016/0022-2836(86)90137-3. View

Bennoun P, Spierer-Herz M, Erickson J, Girard-Bascou J, Pierre Y, Delosme M . Characterization of photosystem II mutants of Chlamydomonas reinhardii lacking the psbA gene. Plant Mol Biol. 2013; 6(3):151-60. DOI: 10.1007/BF00021484. View

Lagoutte B . Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I polypeptide. FEBS Lett. 1988; 232(2):275-80. DOI: 10.1016/0014-5793(88)80752-x. View

Okkels J, Jepsen L, Honberg L, Lehmbeck J, Scheller H, Brandt P . A cDNA clone encoding a 10.8 kDa photosystem I polypeptide of barley. FEBS Lett. 1988; 237(1-2):108-12. DOI: 10.1016/0014-5793(88)80181-9. View

Piccioni R, Bennoun P, Chua N . A nuclear mutant of Chlamydomonas reinhardtii defective in photosynthetic photophosphorylation. Characterization of the algal coupling factor ATPase. Eur J Biochem. 1981; 117(1):93-102. DOI: 10.1111/j.1432-1033.1981.tb06307.x. View

Girard J, Chua N, Bennoun P, Schmidt G, Delosme M . Studies on mutants deficient in the photosystem I reaction centers in Chlamydomonas reinhardtii. Curr Genet. 2013; 2(3):215-21. DOI: 10.1007/BF00435689. View

Hoffman N, Pichersky E, Malik V, Ko K, Cashmore A . Isolation and sequence of a tomato cDNA clone encoding subunit II of the photosystem I reaction center. Plant Mol Biol. 2013; 10(5):435-45. DOI: 10.1007/BF00014949. View

Knaff D . The photosystem I reaction centre. Trends Biochem Sci. 1988; 13(12):460-1. DOI: 10.1016/0968-0004(88)90228-9. View

Kozak M . An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987; 15(20):8125-48. PMC: 306349. DOI: 10.1093/nar/15.20.8125. View

10.

Wynn R, Malkin R . Interaction of plastocyanin with photosystem I: a chemical cross-linking study of the polypeptide that binds plastocyanin. Biochemistry. 1988; 27(16):5863-9. DOI: 10.1021/bi00416a007. View

11.

Keegstra K, Bauerle C . Targeting of proteins into chloroplasts. Bioessays. 1988; 9(1):15-9. DOI: 10.1002/bies.950090105. View

12.

Steppuhn J, Hermans J, Nechushtai R, Ljungberg U, Thummler F, Lottspeich F . Nucleotide sequence of cDNA clones encoding the entire precursor polypeptides for subunits IV and V of the photosystem I reaction center from spinach. FEBS Lett. 1988; 237(1-2):218-24. DOI: 10.1016/0014-5793(88)80205-9. View

13.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

14.

Amati B, Goldschmidt-Clermont M, Wallace C, Rochaix J . cDNA and deduced amino acid sequences of cytochrome c from Chlamydomonas reinhardtii: unexpected functional and phylogenetic implications. J Mol Evol. 1988; 28(1-2):151-60. DOI: 10.1007/BF02143507. View

15.

Bengis C, Nelson N . Subunit structure of chloroplast photosystem I reaction center. J Biol Chem. 1977; 252(13):4564-9. View

16.

Mullet J, Burke J, Arntzen C . Chlorophyll proteins of photosystem I. Plant Physiol. 1980; 65(5):814-22. PMC: 440430. DOI: 10.1104/pp.65.5.814. View

17.

Hoj P, Svendsen I, Scheller H, Moller B . Identification of a chloroplast-encoded 9-kDa polypeptide as a 2[4Fe-4S] protein carrying centers A and B of photosystem I. J Biol Chem. 1987; 262(26):12676-84. View

18.

Lutcke H, Chow K, Mickel F, Moss K, Kern H, SCHEELE G . Selection of AUG initiation codons differs in plants and animals. EMBO J. 1987; 6(1):43-8. PMC: 553354. DOI: 10.1002/j.1460-2075.1987.tb04716.x. View

19.

Goldschmidt-Clermont M . The two genes for the small subunit of RuBP Carboxylase/oxygenase are closely linked in Chlamydomonas reinhardtii. Plant Mol Biol. 2013; 6(1):13-21. DOI: 10.1007/BF00021302. View

20.

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View