Changes in Composition of Membrane Proteins Accompanying the Regulation of PS I/PS II Stoichiometry Observed with Synechocystis PCC 6803

Overview

Journal Photosynth Res

Publisher Springer

Date 2014 Jan 11

PMID 24408283

Citations 7

Authors

K Aizawa

T Shimizu

T Hiyama

K Satoh

Y Nakamura

Affiliations

Soon will be listed here.

Abstract

Changes in composition of membrane proteins in Synechocystis PCC 6803 induced by the shift of light regime for photosynthetic growth were studied in relation to the regulation of PS I/PS II stoichiometry. Special attention was paid to the changes in abundance of proteins of PS I and PS II complexes. Composition was examined using a LDS-PAGE and a quantitative enzyme immunoassay. Abundance of PsaA/B polypeptides and the PsaC polypeptide of the PS I complex, on a per cell basis, increased under the light regime exciting preferentially PS II and decreased under the light regime exciting mainly PS I. Similar changes were observed with polypeptides of 18.5, 10 and 8.5 kDa. The abundance of other proteins associated with membranes, including PsbA polypeptide of the PS II complex, was fairly constant irrespective of light regime. These results are consistent with our previous observations with other strains of cyanophytes (Anabaena variabilis M2 and Synechocystis PCC 6714) that PS I is the variable component in changes in PS I/PS II stoichiometry in response to changing light regimes for photosynthesis.

Citing Articles

Light quality affects chlorophyll biosynthesis and photosynthetic performance in Antarctic Chlamydomonas.

Poirier M, Fugard K, Cvetkovska M Photosynth Res. 2025; 163(1):9.

PMID: 39832016 DOI: 10.1007/s11120-024-01127-0.

Characterization of the wave phenomenon of flash-induced chlorophyll fluorescence in Chlamydomonas reinhardtii.

Patil P, Mohammad Aslam S, Vass I, Szabo M Photosynth Res. 2022; 152(2):235-244.

PMID: 35166999 PMC: 9424139. DOI: 10.1007/s11120-022-00900-3.

Adaptation of light-harvesting functions of unicellular green algae to different light qualities.

Ueno Y, Aikawa S, Kondo A, Akimoto S Photosynth Res. 2018; 139(1-3):145-154.

PMID: 29808364 DOI: 10.1007/s11120-018-0523-y.

Distribution and dynamics of electron transport complexes in cyanobacterial thylakoid membranes.

Liu L Biochim Biophys Acta. 2015; 1857(3):256-65.

PMID: 26619924 PMC: 4756276. DOI: 10.1016/j.bbabio.2015.11.010.

Selective disruption of energy flow from phycobilisomes to Photosystem I.

Glazer A, Gindt Y, Chan C, Sauer K Photosynth Res. 2013; 40(2):167-73.

PMID: 24311285 DOI: 10.1007/BF00019333.

References

Zhao J, Warren P, Li N, Bryant D, Golbeck J . Reconstitution of electron transport in photosystem I with PsaC and PsaD proteins expressed in Escherichia coli. FEBS Lett. 1990; 276(1-2):175-80. DOI: 10.1016/0014-5793(90)80536-r. View

Chitnis P, Purvis D, Nelson N . Molecular cloning and targeted mutagenesis of the gene psaF encoding subunit III of photosystem I from the cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem. 1991; 266(30):20146-51. View

Wynn R, Malkin R . Interaction of plastocyanin with photosystem I: a chemical cross-linking study of the polypeptide that binds plastocyanin. Biochemistry. 1988; 27(16):5863-9. DOI: 10.1021/bi00416a007. View

Domingo A, Marco R . Visualization under ultraviolet light enhances 100-fold the sensitivity of peroxidase-stained blots. Anal Biochem. 1989; 182(1):176-81. DOI: 10.1016/0003-2697(89)90738-0. View

Myers J, Graham J, Wang R . Light Harvesting in Anacystis nidulans Studied in Pigment Mutants. Plant Physiol. 1980; 66(6):1144-9. PMC: 440806. DOI: 10.1104/pp.66.6.1144. View

Hiyama T, Ke B . Difference spectra and extinction coefficients of P 700 . Biochim Biophys Acta. 1972; 267(1):160-71. DOI: 10.1016/0005-2728(72)90147-8. View

Chow W, Melis A, Anderson J . Adjustments of photosystem stoichiometry in chloroplasts improve the quantum efficiency of photosynthesis. Proc Natl Acad Sci U S A. 1990; 87(19):7502-6. PMC: 54775. DOI: 10.1073/pnas.87.19.7502. View

Ley A . Effective Absorption Cross-Sections in Porphyridium cruentum: Implications for Energy Transfer between Phycobilisomes and Photosystem II Reaction Centers. Plant Physiol. 1984; 74(2):451-4. PMC: 1066702. DOI: 10.1104/pp.74.2.451. View

Eichacker L, Soll J, Lauterbach P, Rudiger W, Klein R, Mullet J . In vitro synthesis of chlorophyll a in the dark triggers accumulation of chlorophyll a apoproteins in barley etioplasts. J Biol Chem. 1990; 265(23):13566-71. View

10.

Chitnis P, Reilly P, Miedel M, Nelson N . Structure and targeted mutagenesis of the gene encoding 8-kDa subunit of photosystem I from the cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem. 1989; 264(31):18374-80. View

11.

Garewal H, WASSERMAN A . Triton X-100-4 M urea as an extraction medium for membrane proteins. I. Purification of chloroplast cytochrome b559. Biochemistry. 1974; 13(20):4063-71. DOI: 10.1021/bi00717a001. View

12.

Takabe T, Akazawa T . Biosynthesis of P700-Chlorophyll a Protein Complex, Plastocyanin, and Cytochrome b(6)/f Complex. Plant Physiol. 1986; 81(1):60-6. PMC: 1075283. DOI: 10.1104/pp.81.1.60. View

13.

Klein R, Gamble P, Mullet J . Light-Dependent Accumulation of Radiolabeled Plastid-Encoded Chlorophyll a-Apoproteins Requires Chlorophyll a: I. Analysis of Chlorophyll-Deficient Mutants and Phytochrome Involvement. Plant Physiol. 1988; 88(4):1246-56. PMC: 1055749. DOI: 10.1104/pp.88.4.1246. View

14.

Franzen L, FRANK G, Zuber H, Rochaix J . Isolation and characterization of cDNA clones encoding the 17.9 and 8.1 kDa subunits of Photosystem I from Chlamydomonas reinhardtii. Plant Mol Biol. 2013; 12(4):463-74. DOI: 10.1007/BF00017585. View

15.

Brown R, Jarvis K, Hyland K . Protein measurement using bicinchoninic acid: elimination of interfering substances. Anal Biochem. 1989; 180(1):136-9. DOI: 10.1016/0003-2697(89)90101-2. View

16.

Delepelaire P, Chua N . Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4 degrees C: Characterizations of two additional chlorophyll a-protein complexes. Proc Natl Acad Sci U S A. 1979; 76(1):111-5. PMC: 382886. DOI: 10.1073/pnas.76.1.111. View

17.

Mattoo A, Marder J, Edelman M . Dynamics of the photosystem II reaction center. Cell. 1989; 56(2):241-6. DOI: 10.1016/0092-8674(89)90897-0. View

18.

Reilly P, Hulmes J, Pan Y, Nelson N . Molecular cloning and sequencing of the psaD gene encoding subunit II of photosystem I from the cyanobacterium, Synechocystis sp. PCC 6803. J Biol Chem. 1988; 263(33):17658-62. View

19.

Cunningham F, Dennenberg R, Jursinic P, Gantt E . Growth under Red Light Enhances Photosystem II Relative to Photosystem I and Phycobilisomes in the Red Alga Porphyridium cruentum. Plant Physiol. 1990; 93(3):888-95. PMC: 1062605. DOI: 10.1104/pp.93.3.888. View

20.

Koike H, Ikeuchi M, Hiyama T, Inoue Y . Identification of photosystem I components from the cyanobacterium, Synechococcus vulcanus by N-terminal sequencing. FEBS Lett. 1989; 253(1-2):257-63. DOI: 10.1016/0014-5793(89)80971-8. View