Homologous and Heterologous Protein Import into Mitochondria Isolated from the Green Alga Chlamydomonas Reinhardtii

Overview

Journal Plant Mol Biol

Date 1998 Jan 14

PMID 9426617

Citations 4

Authors

G Nurani

M Eriksson

C Knorpp

E Glaser

L G Franzen

Affiliations

Soon will be listed here.

Abstract

We have established a homologous system for studying mitochondrial protein import in Chlamydomonas reinhardtii, using C. reinhardtii precursor proteins and mitochondria isolated from C. reinhardtii. The precursors of the F1 alpha ATP synthase subunit and the Rieske FeS protein were imported into mitochondria with high efficiency, while the F1 beta subunit precursor was imported with much lower efficiency. The import of heterologous precursor proteins from higher plants was also less efficient. The precursor of the C. reinhardtii PsaF chloroplast protein was converted into a protease-protected form upon incubation with mitochondria. In vitro processing studies revealed that in contrast to the situation in higher plants, the processing of the precursors was catalysed by a soluble, matrix-located peptidase.

Citing Articles

Reconstructing the mitochondrial protein import machinery of Chlamydomonas reinhardtii.

Figueroa-Martinez F, Funes S, Franzen L, Gonzalez-Halphen D Genetics. 2008; 179(1):149-55.

PMID: 18493047 PMC: 2390594. DOI: 10.1534/genetics.108.087965.

Identification of novel mitochondrial protein components of Chlamydomonas reinhardtii. A proteomic approach.

van Lis R, Atteia A, Mendoza-Hernandez G, Gonzalez-Halphen D Plant Physiol. 2003; 132(1):318-30.

PMID: 12746537 PMC: 166977. DOI: 10.1104/pp.102.018325.

Structure, organization and expression of the genes encoding mitochondrial cytochrome c(1) and the Rieske iron-sulfur protein in Chlamydomonas reinhardtii.

Atteia A, van Lis R, Wetterskog D, Gutierrez-Cirlos E, Ongay-Larios L, Franzen L Mol Genet Genomics. 2003; 268(5):637-44.

PMID: 12589438 DOI: 10.1007/s00438-002-0779-x.

Mitochondrial protein import in plants. Signals, sorting, targeting, processing and regulation.

Glaser E, Sjoling S, Tanudji M, Whelan J Plant Mol Biol. 1998; 38(1-2):311-38.

PMID: 9738973 DOI: 10.1023/a:1006020208140.

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