Genetic Analysis of G12P[8] Rotaviruses Detected in the Largest U.S. G12 Genotype Outbreak on Record

Overview

Journal Infect Genet Evol

Specialties Biology
Genetics
Infectious Diseases

Date 2013 Nov 26

PMID 24270016

Citations 13

Authors

Slavica Mijatovic-Rustempasic

Elizabeth N Teel

Tara K Kerin

Jennifer J Hull

Sunando Roy

Geoffrey A Weinberg

Daniel C Payne

Umesh D Parashar

Jon R Gentsch

Michael D Bowen

Affiliations

Soon will be listed here.

Abstract

In 2006-07, 77 cases of gastroenteritis in Rochester, NY, USA were associated with rotavirus genotype G12P[8]. Sequence analysis identified a high degree of genetic relatedness among the VP7 and VP4 genes of the Rochester G12P[8] strains and between these strains and currently circulating human G12P[8] strains. Out of 77 samples, two and seven unique nucleotide sequences were identified for VP7 and VP4 genes, respectively. Rochester strain VP7 genes were found to occupy the G12-III lineage and VP4 genes clustered within the P[8]-3 lineage. Six strains contained non-synonymous nucleotide substitutions that produced amino acid changes at 6 sites in the VP8(∗) region of the VP4 gene. Two sites (amino acids 242 and 246) were located in or near a described trypsin cleavage site. Selection analyses identified one positively selected VP7 site (107) and strong purifying selection at 58 sites within the VP7 gene as well as 2 of the 6 variant sites (79 and 218) in VP4.

Citing Articles

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