Molecular Markers (RFLPs and HSPs) for the Genetic Dissection of Thermotolerance in Maize

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 14

PMID 24221430

Citations 17

Authors

E Ottaviano

M Sari Gorla

E Pe

C Frova

Affiliations

Soon will be listed here.

Abstract

Cellular membrane stability (CMS) is a physiological index widely used to evaluate thermostability in plants. The genetic basis of the character has been studied following two different approaches: restriction fragment length polymorphism (RFLP) analysis, and the effects of segregating heat shock protein (HSP) loci. RFLP analysis was based on a set of recombinant inbreds derived from the T32 × CM37 F1 hybrid and characterized for about 200 RFLP loci. Heritability of CMS estimated by standard quantitative analysis was 0.73. Regression analysis of CMS on RFLPs detected a minimum number of six quantitative trait loci (QTL) accounting for 53% of the genetic variability. The analysis of the matrices of correlation between RFLP loci, either within or between chromosomes, indicates that no false assignment was produced by this analysis. The effect of HSPs on the variability of the CMS was tested for a low-molecular-weight peptide (HSP-17) showing presence-absence of segregation in the B73 × Pa33 F2 population. Although the genetic variability of the character was very high (h (2)=0.58) the effect of HSP-17 was not significant, indicating either that the polypeptide is not involved in the determination of the character or that its effect is not statistically detectable.

Citing Articles

Genes and pathways correlated with heat stress responses and heat tolerance in maize kernels.

Chen Y, Du T, Zhang J, Chen S, Fu J, Li H Front Plant Sci. 2023; 14:1228213.

PMID: 37662159 PMC: 10470023. DOI: 10.3389/fpls.2023.1228213.

Insights into morphological and physio-biochemical adaptive responses in mungbean ( L.) under heat stress.

Bhardwaj R, Lone J, Pandey R, Mondal N, Dhandapani R, Meena S Front Genet. 2023; 14:1206451.

PMID: 37396038 PMC: 10308031. DOI: 10.3389/fgene.2023.1206451.

QTL mapping and genome-wide prediction of heat tolerance in multiple connected populations of temperate maize.

Van Inghelandt D, Frey F, Ries D, Stich B Sci Rep. 2019; 9(1):14418.

PMID: 31594984 PMC: 6783442. DOI: 10.1038/s41598-019-50853-2.

First steps to understand heat tolerance of temperate maize at adult stage: identification of QTL across multiple environments with connected segregating populations.

Frey F, Presterl T, Lecoq P, Orlik A, Stich B Theor Appl Genet. 2016; 129(5):945-61.

PMID: 26886101 PMC: 4835532. DOI: 10.1007/s00122-016-2674-6.

Mapping QTL for the traits associated with heat tolerance in wheat (Triticum aestivum L.).

Talukder S, Babar M, Vijayalakshmi K, Poland J, Prasad P, Bowden R BMC Genet. 2014; 15:97.

PMID: 25384418 PMC: 4234900. DOI: 10.1186/s12863-014-0097-4.

References

Zivy M . Genetic variability for heat shock proteins in common wheat. Theor Appl Genet. 2013; 74(2):209-13. DOI: 10.1007/BF00289970. View

Martin B, Nienhuis J, King G, Schaefer A . Restriction fragment length polymorphisms associated with water use efficiency in tomato. Science. 1989; 243(4899):1725-8. DOI: 10.1126/science.243.4899.1725. View

Weller J, Soller M, Brody T . Linkage analysis of quantitative traits in an interspecific cross of tomato (lycopersicon esculentum x lycopersicon pimpinellifolium) by means of genetic markers. Genetics. 1988; 118(2):329-39. PMC: 1203285. DOI: 10.1093/genetics/118.2.329. View

Krishnan M, Nguyen H, Burke J . Heat shock protein synthesis and thermal tolerance in wheat. Plant Physiol. 1989; 90(1):140-5. PMC: 1061688. DOI: 10.1104/pp.90.1.140. View

Zamir D, Tanksley S, Jones R . Haploid selection for low temperature tolerance of tomato pollen. Genetics. 1982; 101(1):129-37. PMC: 1201846. DOI: 10.1093/genetics/101.1.129. View

Osborn T, Alexander D, Fobes J . Identification of restriction fragment length polymorphisms linked to genes controlling soluble solids content in tomato fruit. Theor Appl Genet. 2013; 73(3):350-6. DOI: 10.1007/BF00262500. View

Burr B, Burr F, Thompson K, Albertson M, Stuber C . Gene mapping with recombinant inbreds in maize. Genetics. 1988; 118(3):519-26. PMC: 1203305. DOI: 10.1093/genetics/118.3.519. View

Taylor B . Genetic analysis of susceptibility to isoniazid-induced seizures in mice. Genetics. 1976; 83(2):373-7. PMC: 1213520. DOI: 10.1093/genetics/83.2.373. View

Lindquist S . The heat-shock response. Annu Rev Biochem. 1986; 55:1151-91. DOI: 10.1146/annurev.bi.55.070186.005443. View

10.

Edwards M, Stuber C, Wendel J . Molecular-marker-facilitated investigations of quantitative-trait loci in maize. I. Numbers, genomic distribution and types of gene action. Genetics. 1987; 116(1):113-25. PMC: 1203110. DOI: 10.1093/genetics/116.1.113. View

11.

Kahler A, Wehrhahn C . Associations between quantitative traits and enzyme loci in the F2 population of a maize hybrid. Theor Appl Genet. 2013; 72(1):15-26. DOI: 10.1007/BF00261448. View

12.

Lin C, Roberts J, Key J . Acquisition of Thermotolerance in Soybean Seedlings : Synthesis and Accumulation of Heat Shock Proteins and their Cellular Localization. Plant Physiol. 1984; 74(1):152-60. PMC: 1066642. DOI: 10.1104/pp.74.1.152. View

13.

Mansfield M, Key J . Synthesis of the low molecular weight heat shock proteins in plants. Plant Physiol. 1987; 84(4):1007-17. PMC: 1056718. DOI: 10.1104/pp.84.4.1007. View

14.

Paterson A, Lander E, Hewitt J, Peterson S, Lincoln S, Tanksley S . Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature. 1988; 335(6192):721-6. DOI: 10.1038/335721a0. View