Similarity of Maize and Sorghum Genomes As Revealed by Maize RFLP Probes

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 9

PMID 24203022

Citations 21

Authors

G Binelli

L Gianfranceschi

M E Pe

G Taramino

C Busso

J Stenhouse

E Ottaviano

Affiliations

Soon will be listed here.

Abstract

Densely saturated genetic maps of neutral genetic markers are a prerequisite either for plant breeding programs to improve quantitative traits in crops or for evolutionary studies. cDNA and genomic clones from maize were utilized to initiate the construction of a RFLP linkage map in Sorghum bicolor. To this purpose, an F2 population was produced from starting parental lines IS 18729 (USA) and IS 24756 (Nigeria) that were differentiated with regard to many morphological and agronomical traits. A total of 159 maize clones were hybridized to the genomic DNA of the two parents in order to detect polymorphism: 154 probes hybridized to sorghum and 58 out of these were polymorphic. In almost all of the cases hybridization patterns were similar between maize and sorghum. The analysis of the segregation of 35 polymorphic clones in an F2 population of 149 individuals yielded five linkage groups. The three principal ones recall regions of maize chromosomes 1, 3 and 5: in general, colinearity was maintained. A possible inversion, involving a long region of maize chromosome 3, was detected. Simulations were also performed to empirically obtain a value for the lowest number of individuals of the F2 population needed to obtain the same linkage data.

Citing Articles

Recent advancements in the breeding of sorghum crop: current status and future strategies for marker-assisted breeding.

Baloch F, Altaf M, Liaqat W, Bedir M, Nadeem M, Comertpay G Front Genet. 2023; 14:1150616.

PMID: 37252661 PMC: 10213934. DOI: 10.3389/fgene.2023.1150616.

Comparative genome analysis of mungbean (Vigna radiata L. Wilczek) and cowpea (V. unguiculata L. Walpers) using RFLP mapping data.

Menancio-Hautea D, Fatokun C, Kumar L, Danesh D, Young N Theor Appl Genet. 2013; 86(7):797-810.

PMID: 24193874 DOI: 10.1007/BF00212605.

Structure and evolution of the genomes ofsorghum bicolor andZea mays.

Berhan A, Hulbert S, Butler L, Bennetzen J Theor Appl Genet. 2013; 86(5):598-604.

PMID: 24193709 DOI: 10.1007/BF00838715.

A detailed RFLP map of Sorghum bicolor x S. propinquum, suitable for high-density mapping, suggests ancestral duplication of Sorghum chromosomes or chromosomal segments.

Chittenden L, Schertz K, Lin Y, Wing R, Paterson A Theor Appl Genet. 2013; 87(8):925-33.

PMID: 24190526 DOI: 10.1007/BF00225786.

Genetic diversity among elite Sorghum lines revealed by restriction fragment length polymorphisms and random amplified polymorphic DNAs.

Vierling R, Xiang Z, Joshi C, Gilbert M, Nguyen H Theor Appl Genet. 2013; 87(7):816-20.

PMID: 24190467 DOI: 10.1007/BF00221133.

References

Ottaviano E, Sari Gorla M, Pe E, Frova C . Molecular markers (RFLPs and HSPs) for the genetic dissection of thermotolerance in maize. Theor Appl Genet. 2013; 81(6):713-9. DOI: 10.1007/BF00224979. View

Welsh J, McClelland M . Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990; 18(24):7213-8. PMC: 332855. DOI: 10.1093/nar/18.24.7213. View

Beckmann J, Soller M . Detection of linkage between marker loci and loci affecting quantitative traits in crosses between segregating populations. Theor Appl Genet. 2013; 76(2):228-36. DOI: 10.1007/BF00257850. View

Rees H, Durrant A . Recombination and genome size. Theor Appl Genet. 2013; 73(1):72-6. DOI: 10.1007/BF00273721. View

Hulbert S, Richter T, Axtell J, Bennetzen J . Genetic mapping and characterization of sorghum and related crops by means of maize DNA probes. Proc Natl Acad Sci U S A. 1990; 87(11):4251-5. PMC: 54086. DOI: 10.1073/pnas.87.11.4251. View

Debener T, Salamini F, Gebhardt C . Phylogeny of wild and cultivatedSolanum species based on nuclear restriction fragment length polymorphisms (RFLPs). Theor Appl Genet. 2013; 79(3):360-8. DOI: 10.1007/BF01186080. View

Syvanen M, Hartman H, Stevens P . Classical plant taxonomic ambiguities extend to the molecular level. J Mol Evol. 1989; 28(6):536-44. DOI: 10.1007/BF02602934. View

G Williams J, Kubelik A, Livak K, Rafalski J, Tingey S . DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990; 18(22):6531-5. PMC: 332606. DOI: 10.1093/nar/18.22.6531. View

Miller J, Tanksley S . RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet. 2013; 80(4):437-48. DOI: 10.1007/BF00226743. View

10.

Bernatzky R, Tanksley S . Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics. 1986; 112(4):887-98. PMC: 1202783. DOI: 10.1093/genetics/112.4.887. View

11.

Paterson A, Lander E, Hewitt J, Peterson S, Lincoln S, Tanksley S . Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature. 1988; 335(6192):721-6. DOI: 10.1038/335721a0. View

12.

Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H . RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet. 2013; 78(1):65-75. DOI: 10.1007/BF00299755. View

13.

Helentjaris T, Slocum M, Wright S, Schaefer A, Nienhuis J . Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Theor Appl Genet. 2013; 72(6):761-9. DOI: 10.1007/BF00266542. View

14.

Ritter E, Gebhardt C, Salamini F . Estimation of recombination frequencies and construction of RFLP linkage maps in plants from crosses between heterozygous parents. Genetics. 1990; 125(3):645-54. PMC: 1204090. DOI: 10.1093/genetics/125.3.645. View

15.

Pichersky E, Bernatzky R, Tanksley S, Cashmore A . Evidence for selection as a mechanism in the concerted evolution of Lycopersicon esculentum (tomato) genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci U S A. 1986; 83(11):3880-4. PMC: 323628. DOI: 10.1073/pnas.83.11.3880. View

16.

McCouch S, Kochert G, Yu Z, Wang Z, Khush G, Coffman W . Molecular mapping of rice chromosomes. Theor Appl Genet. 2013; 76(6):815-29. DOI: 10.1007/BF00273666. View

17.

Martin G, G Williams J, Tanksley S . Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci U S A. 1991; 88(6):2336-40. PMC: 51226. DOI: 10.1073/pnas.88.6.2336. View

18.

Helentjaris T, Weber D, Wright S . Identification of the genomic locations of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms. Genetics. 1988; 118(2):353-63. PMC: 1203287. DOI: 10.1093/genetics/118.2.353. View

19.

Burr B, Burr F, Thompson K, Albertson M, Stuber C . Gene mapping with recombinant inbreds in maize. Genetics. 1988; 118(3):519-26. PMC: 1203305. DOI: 10.1093/genetics/118.3.519. View

20.

Soller M, Beckmann J . Genetic polymorphism in varietal identification and genetic improvement. Theor Appl Genet. 2013; 67(1):25-33. DOI: 10.1007/BF00303917. View