Genetic Variability for Heat Shock Proteins in Common Wheat

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 19

PMID 24241566

Citations 7

Authors

M Zivy

Affiliations

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Abstract

The response of the common wheat line 'Chinese Spring' to heat shocks of different time lengths was studied by the two-dimensional (2D) electrophoresis of denatured proteins. After a heat shock of 5 h, 33 heat shock proteins (HSPs) accumulated in an amount sufficient to be revealed by silver stain. Two other wheat lines ('Moisson' and 'Selkirk') were then submitted to a heat shock of 5 h, and the responses of the 3 lines were compared: of a total of 35 HSPs, 13 (37.1%) were quantitatively or qualitatively variable. This variability concerns low-molecular-weight and high-molecular-weight HSPs. The three genotypes showed thermal tolerance but 'Chinese Spring's' response to heat treatments was slightly different from those of the other two lines The possibility of a relationship between HSP patterns and thermal sensitivity is discussed.

Citing Articles

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References

Colas des Francs C, Thiellement H . Chromosomal localization of structural genes and regulators in wheat by 2D electrophoresis of ditelosomic lines. Theor Appl Genet. 2013; 71(1):31-8. DOI: 10.1007/BF00278250. View

McAlister L, Finkelstein D . Heat shock proteins and thermal resistance in yeast. Biochem Biophys Res Commun. 1980; 93(3):819-24. DOI: 10.1016/0006-291x(80)91150-x. View

Cooper P, Ho T, Hauptmann R . Tissue specificity of the heat-shock response in maize. Plant Physiol. 1984; 75(2):431-41. PMC: 1066925. DOI: 10.1104/pp.75.2.431. View

Zivy M, Thiellement H, de Vienne D, Hofmann J . Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis : 2. Genetic differences between two lines and two groups of cytoplasms at five developmental stages or organs. Theor Appl Genet. 2013; 68(4):335-45. DOI: 10.1007/BF00267887. View

Vierling E, Mishkind M, Schmidt G, Key J . Specific heat shock proteins are transported into chloroplasts. Proc Natl Acad Sci U S A. 1986; 83(2):361-5. PMC: 322858. DOI: 10.1073/pnas.83.2.361. View

Granier F, de Vienne D . Silver staining of proteins: standardized procedure for two-dimensional gels bound to polyester sheets. Anal Biochem. 1986; 155(1):45-50. DOI: 10.1016/0003-2697(86)90222-8. View

Lin C, Roberts J, Key J . Acquisition of Thermotolerance in Soybean Seedlings : Synthesis and Accumulation of Heat Shock Proteins and their Cellular Localization. Plant Physiol. 1984; 74(1):152-60. PMC: 1066642. DOI: 10.1104/pp.74.1.152. View

Zimmerman J, Petri W, Meselson M . Accumulation of a specific subset of D. melanogaster heat shock mRNAs in normal development without heat shock. Cell. 1983; 32(4):1161-70. DOI: 10.1016/0092-8674(83)90299-4. View

Velazquez J, DiDomenico B, Lindquist S . Intracellular localization of heat shock proteins in Drosophila. Cell. 1980; 20(3):679-89. DOI: 10.1016/0092-8674(80)90314-1. View

10.

Moran L, Chauvin M, Kennedy M, Korri M, Lowe D, Nicholson R . The major heat-shock protein (hsp70) gene family: related sequences in mouse, Drosophila, and yeast. Can J Biochem Cell Biol. 1983; 61(6):488-99. DOI: 10.1139/o83-065. View

11.

Loomis W, Wheeler S . Chromatin-associated heat shock proteins of Dictyostelium. Dev Biol. 1982; 90(2):412-8. DOI: 10.1016/0012-1606(82)90390-6. View

12.

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

13.

Yamamori T, Yura T . Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1982; 79(3):860-4. PMC: 345852. DOI: 10.1073/pnas.79.3.860. View

14.

Oakley B, Kirsch D, Morris N . A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980; 105(2):361-3. DOI: 10.1016/0003-2697(80)90470-4. View

15.

Lopato S, Gleba Y . Heat shock proteins from cell cultures of higher plants and their somatic hybrids. Plant Cell Rep. 2013; 4(1):19-22. DOI: 10.1007/BF00285496. View

16.

Kee S, Nobel P . Concomitant changes in high temperature tolerance and heat-shock proteins in desert succulents. Plant Physiol. 1986; 80(2):596-8. PMC: 1075161. DOI: 10.1104/pp.80.2.596. View

17.

Zannis V, Breslow J . Human very low density lipoprotein apolipoprotein E isoprotein polymorphism is explained by genetic variation and posttranslational modification. Biochemistry. 1981; 20(4):1033-41. DOI: 10.1021/bi00507a059. View

18.

Kelley P, SCHLESINGER M . Antibodies to two major chicken heat shock proteins cross-react with similar proteins in widely divergent species. Mol Cell Biol. 1982; 2(3):267-74. PMC: 369785. DOI: 10.1128/mcb.2.3.267-274.1982. View