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Subcellular Localization of Enzymes Involved in Indole Alkaloid Biosynthesis in Catharanthus Roseus

Overview
Journal Plant Physiol
Specialty Physiology
Date 1987 Dec 1
PMID 16665811
Citations 29
Authors
V De Luca
A J Cutler
Affiliations
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Abstract

The subcellular localization of enzymes involved in indole alkaloid biosynthesis in leaves of Catharanthus roseus has been investigated. Tryptophan decarboxylase and strictosidine synthase which together produce strictosidine, the first indole alkaloid of this pathway, are both cytoplasmic enzymes. S-Adenosyl-l-methionine: 16-methoxy-2,3-dihydro-3-hydroxytabersonine-N-methyltransferase which catalyses the third to last step in vindoline biosynthesis could be localized in the chloroplasts of Catharanthus leaves and is specifically associated with thylakoids. Acetyl-coenzyme-A-deacetylvindoline-O-acetyltransferase which catalyses the last step in vindoline biosynthesis could also be localized in the cytoplasm. The participation of the chloroplast in this pathway suggests that indole alkaloid intermediates enter and exit this compartment during the biosynthesis of vindoline.

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References
1.
Madyastha K, Guarnaccia R, Baxter C, Coscia C . S-Adenosyl-L-methionine: loganic acid methyltransferase. A carboxyl-alkylating enzyme from Vinca rosea. J Biol Chem. 1973; 248(7):2497-501. View
2.
Schwitzguebel J, Siegenthaler P . Purification of peroxisomes and mitochondria from spinach leaf by percoll gradient centrifugation. Plant Physiol. 1984; 75(3):670-4. PMC: 1066974. DOI: 10.1104/pp.75.3.670. View
3.
Engvall E . Enzyme immunoassay ELISA and EMIT. Methods Enzymol. 1980; 70(A):419-39. DOI: 10.1016/s0076-6879(80)70067-8. View
4.
Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View
5.
Wink M, Hartmann T . Localization of the Enzymes of Quinolizidine Alkaloid Biosynthesis in Leaf Chloroplasts of Lupinus polyphyllus. Plant Physiol. 1982; 70(1):74-7. PMC: 1067088. DOI: 10.1104/pp.70.1.74. View