A CREB3-ARF4 Signalling Pathway Mediates the Response to Golgi Stress and Susceptibility to Pathogens

Overview

Journal Nat Cell Biol

Specialty Cell Biology

Date 2013 Nov 5

PMID 24185178

Citations 93

Authors

Jan H Reiling

Andrew J Olive

Sumana Sanyal

Jan E Carette

Thijn R Brummelkamp

Hidde L Ploegh

Michael N Starnbach

David M Sabatini

Affiliations

Soon will be listed here.

Abstract

Treatment of cells with brefeldin A (BFA) blocks secretory vesicle transport and causes a collapse of the Golgi apparatus. To gain more insight into the cellular mechanisms mediating BFA toxicity, we conducted a genome-wide haploid genetic screen that led to the identification of the small G protein ADP-ribosylation factor 4 (ARF4). ARF4 depletion preserves viability, Golgi integrity and cargo trafficking in the presence of BFA, and these effects depend on the guanine nucleotide exchange factor GBF1 and other ARF isoforms including ARF1 and ARF5. ARF4 knockdown cells show increased resistance to several human pathogens including Chlamydia trachomatis and Shigella flexneri. Furthermore, ARF4 expression is induced when cells are exposed to several Golgi-disturbing agents and requires the CREB3 (also known as Luman or LZIP) transcription factor, whose downregulation mimics ARF4 loss. Thus, we have uncovered a CREB3-ARF4 signalling cascade that may be part of a Golgi stress response set in motion by stimuli compromising Golgi capacity.

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