Rat Aldolase A Messenger RNA: the Nucleotide Sequence and Multiple MRNA Species with Different 5'-terminal Regions

Overview

Journal Gene

Specialty Molecular Biology

Date 1985 Jan 1

PMID 2416636

Citations 6

Authors

K Joh

T Mukai

H Yatsuki

K Hori

Affiliations

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Abstract

The nucleotide sequence of aldolase A mRNA in rat skeletal muscle was determined using recombinant cDNA clones and a cDNA synthesized by primer extension. The sequence is composed of 1343 nucleotides (nt) except for the poly(A) tail. Based on the sequence analysis we have deduced an open reading frame with 363 amino acids (aa) (Mr 39134). The sequence suggests several nt polymorphisms in the mRNA population, one of which causes an aa change. The determined sequence of rat aldolase A mRNA was compared with the published ones of rabbit aldolase A or rat aldolase B mRNAs. The homology between rat and rabbit aldolase A mRNA sequences is greater than that between rat aldolase A and B mRNA sequences. Multiple aldolase A mRNAs having different Mrs were detected in the various tissues, and appeared to be expressed in a tissue-specific manner. Further analysis suggests that differences in mRNA length are due to differences in the 5'-noncoding terminal region.

Citing Articles

Molecular gene mapping of human aldolase A (ALDOA) gene to chromosome 16.

Kukita A, Yoshida M, Fukushige S, Sakakibara M, Joh K, Mukai T Hum Genet. 1987; 76(1):20-6.

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Sequence analysis of the cDNA encoding human liver glycogen phosphorylase reveals tissue-specific codon usage.

Newgard C, Nakano K, Hwang P, Fletterick R Proc Natl Acad Sci U S A. 1986; 83(21):8132-6.

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Localization of the active gene of aldolase on chromosome 16, and two aldolase A pseudogenes on chromosomes 3 and 10.

Serero S, Maire P, Nguyen V, Cohen-Haguenauer O, Gross M, De Tand M Hum Genet. 1988; 78(2):167-74.

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Human aldolase A deficiency associated with a hemolytic anemia: thermolabile aldolase due to a single base mutation.

Kishi H, Mukai T, Hirono A, Fujii H, Miwa S, Hori K Proc Natl Acad Sci U S A. 1987; 84(23):8623-7.

PMID: 2825199 PMC: 299598. DOI: 10.1073/pnas.84.23.8623.

Nucleic acid composition, codon usage, and the rate of synonymous substitution in protein-coding genes.

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