Localization of the Active Gene of Aldolase on Chromosome 16, and Two Aldolase A Pseudogenes on Chromosomes 3 and 10

Overview

Journal Hum Genet

Specialty Genetics

Date 1988 Feb 1

PMID 2828224

Citations 1

Authors

S Serero

P Maire

V C Nguyen

O Cohen-Haguenauer

M S Gross

M F De Tand

A Kahn

J FREZAL

Affiliations

Soon will be listed here.

Abstract

Southern blot analysis of human genomic DNA hybridized with a coding region aldolase A cDNA probe (600 bases) revealed four restriction fragments with EcoRI restriction enzyme: 7.8 kb, 13 kb, 17 kb and greater than 30 kb. By human-hamster hybrid analysis (Southern technique) the principal fragments, 7.8 kb, 13 kb, greater than 30 kb, were localized to chromosomes 10, 16 and 3 respectively. The 17-kb fragment was very weak in intensity; it co-segregated with the greater than 30-kb fragment and is probably localized on chromosome 3 with the greater than 30-kb fragment. Analysis of a second aldolase A labelled probe protected against S1 nuclease digestion by RNAs from different hybrid cells, indicated the presence of aldolase A mRNAs in hybrid cells containing only chromosome 16. Under the stringency conditions used, the EcoRI sequences detected by the coding region aldolase A cDNA probe did not correspond to aldolase B or C. The 7.8-kb and greater than 30-kb EcoRI sequences, localized respectively on chromosomes 10 and 3, correspond to aldolase A pseudogenes; the 13-kb EcoRI sequence localized on chromosome 16 corresponds to the aldolase active gene. The fact that the aldolase A gene and pseudogenes are located on three different chromosomes supports the hypothesis that the pseudogenes originated from aldolase A mRNAs, copied into DNA and integrated in unrelated chromosomal loci.

Citing Articles

Assignment of human aldolase C gene to chromosome 17, region cen----q21.1.

Rocchi M, Vitale E, Covone A, Romeo G, Santamaria R, Buono P Hum Genet. 1989; 82(3):279-82.

PMID: 2731939 DOI: 10.1007/BF00291170.

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