Transcriptional Regulation of an Aldolase Gene in the Regenerating Rat Liver

Overview

Journal Gastroenterol Jpn

Specialty Gastroenterology

Date 1990 Jun 1

PMID 2358166

Authors

M Motomura

T Mukai

I Ozaki

K Joh

Y Arai

T Sakai

K Hori

Affiliations

Soon will be listed here.

Abstract

The relative abundance of rat aldolase A, B, C, alpha-fetoprotein, and albumin mRNAs was determined by Northern hybridization during liver regeneration after partial hepatectomy. Aldolase A mRNA increased more than 10-fold on the 3rd day after partial resection compared with that of normal adult rat liver. S1 analysis revealed that three species of aldolase A mRNAs (mRNA I, II & III) reappeared. However, transcriptional rate of aldolase A mRNA did not change at all during the regeneration. In contrast, aldolase B, aldolase C and albumin mRNAs did not change at all. These findings suggest that the differentiated hepatocyte maintains a differentiated state during the liver regeneration as seen in aldolase B, whereas "oncofetal" isozymes such as aldolase A resurge after partial hepatectomy under the control of post-transcriptional mechanism.

References

Joh K, Arai Y, Mukai T, Hori K . Expression of three mRNA species from a single rat aldolase A gene, differing in their 5' non-coding regions. J Mol Biol. 1986; 190(3):401-10. DOI: 10.1016/0022-2836(86)90011-2. View

Huber B, Heilman C, Wirth P, Miller M, Thorgeirsson S . Studies of gene transcription and translation in regenerating rat liver. Hepatology. 1986; 6(2):209-19. DOI: 10.1002/hep.1840060210. View

Kukita A, Mukai T, Miyata T, Hori K . The structure of brain-specific rat aldolase C mRNA and the evolution of aldolase isozyme genes. Eur J Biochem. 1988; 171(3):471-8. DOI: 10.1111/j.1432-1033.1988.tb13813.x. View

SCHAPIRA F . Resurgence of fetal isozymes in cancer: study of aldolase, pyruvate kinase, lactic dehydrogenase, and beta-hexosaminidase. Isozymes Curr Top Biol Med Res. 1981; 5:27-75. View

BUCHER N . Experimental aspects of hepatic regeneration. N Engl J Med. 1967; 277(14):738-46 concl. DOI: 10.1056/NEJM196710052771405. View

Petropoulos C, Andrews G, Tamaoki T, Fausto N . alpha-Fetoprotein and albumin mRNA levels in liver regeneration and carcinogenesis. J Biol Chem. 1983; 258(8):4901-6. View

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

Tsutsumi K, Mukai T, Tsutsumi R, Mori M, Daimon M, Tanaka T . Nucleotide sequence of rat liver aldolase B messenger RNA. J Biol Chem. 1984; 259(23):14572-5. View

Matsuhashi S, Mukai T, Hori K . Determination of protein spots separated by two-dimensional polyacrylamide gel electrophoresis. J Chromatogr. 1985; 319(1):79-89. DOI: 10.1016/s0021-9673(01)90541-2. View

10.

Mukai T, Joh K, Miyahara H, Sakakibara M, Arai Y, Hori K . Different expression of rat aldolase A mRNA in the skeletal muscle and ascites hepatoma cells. Biochem Biophys Res Commun. 1984; 119(2):575-81. DOI: 10.1016/s0006-291x(84)80287-9. View

11.

Mukai T, Joh K, Arai Y, Yatsuki H, Hori K . Tissue-specific expression of rat aldolase A mRNAs. Three molecular species differing only in the 5'-terminal sequences. J Biol Chem. 1986; 261(7):3347-54. View

12.

Weber A, Hatzfeld A, Guillouzo A, SCHAPIRA F . Cell localization of aldolase fetal isozymes in rat regenerating liver: differences with hepatoma. Biochem Biophys Res Commun. 1979; 86(1):6-13. DOI: 10.1016/0006-291x(79)90374-7. View

13.

BUCHER N . Experimental aspects of hepatic regeneration. N Engl J Med. 1967; 277(13):686-96 contd. DOI: 10.1056/NEJM196709282771306. View

14.

Numazaki M, Tsutsumi K, Tsutsumi R, Ishikawa K . Expression of aldolase isozyme mRNAs in fetal rat liver. Eur J Biochem. 1984; 142(1):165-70. DOI: 10.1111/j.1432-1033.1984.tb08265.x. View

15.

Tsutsumi K, Mukai T, Tsutsumi R, Hidaka S, Arai Y, Hori K . Structure and genomic organization of the rat aldolase B gene. J Mol Biol. 1985; 181(2):153-60. DOI: 10.1016/0022-2836(85)90081-6. View

16.

Friedman J, Chung E, Darnell Jr J . Gene expression during liver regeneration. J Mol Biol. 1984; 179(1):37-53. DOI: 10.1016/0022-2836(84)90305-x. View

17.

Joh K, Mukai T, Yatsuki H, Hori K . Rat aldolase A messenger RNA: the nucleotide sequence and multiple mRNA species with different 5'-terminal regions. Gene. 1985; 39(1):17-24. DOI: 10.1016/0378-1119(85)90102-7. View

18.

Makino R, Hayashi K, Sugimura T . C-myc transcript is induced in rat liver at a very early stage of regeneration or by cycloheximide treatment. Nature. 1984; 310(5979):697-8. DOI: 10.1038/310697a0. View

19.

Rutter W, BLOSTEIN R, WOODFIN B, Weber C . ENZYME VARIANTS AND METABOLIC DIVERSIFICATION. Adv Enzyme Regul. 1963; 1:39-56. DOI: 10.1016/0065-2571(63)90005-0. View

20.

Urano Y, Sakai M, Watanabe K, Tamaoki T . Tandem arrangement of the albumin and alpha-fetoprotein genes in the human genome. Gene. 1984; 32(3):255-61. DOI: 10.1016/0378-1119(84)90001-5. View