Expression of Plant Sweet Protein Brazzein in the Milk of Transgenic Mice

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Oct 25

PMID 24155905

Citations 7

Authors

Sen Yan

Hong Song

Daxin Pang

Qingjian Zou

Li Li

Quanmei Yan

Nana Fan

Xiangjie Zhao

Hao Yu

Zhanjun Li

Haijun Wang

Fei Gao

Hongsheng Ouyang

Liangxue Lai

Affiliations

Soon will be listed here.

Abstract

Sugar, the most popular sweetener, is essential in daily food. However, excessive sugar intake has been associated with several lifestyle-related diseases. Finding healthier and more economical alternatives to sugars and artificial sweeteners has received increasing attention to fulfill the growing demand. Brazzein, which comes from the pulp of the edible fruit of the African plant Pentadiplandra brazzeana Baill, is a protein that is 2,000 times sweeter than sucrose by weight. Here we report the production of transgenic mice that carry the optimized brazzein gene driven by the goat Beta-casein promoter, which specifically directs gene expression in the mammary glands. Using western blot analysis and immunohistochemistry, we confirmed that brazzein could be efficiently expressed in mammalian milk, while retaining its sweetness. This study presents the possibility of producing plant protein-sweetened milk from large animals such as cattle and goats.

Citing Articles

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Brazzein and Monellin: Chemical Analysis, Food Industry Applications, Safety and Quality Control, Nutritional Profile and Health Impacts.

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Expression of a triple mutational des-pGlu brazzein in transgenic mouse milk.

Lu R, Li X, Hu J, Zhang Y, Wang Y, Jin L FEBS Open Bio. 2022; 12(7):1336-1343.

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Efficient brazzein production in yeast (Kluyveromyces lactis) using a chemically defined medium.

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