Efficient Brazzein Production in Yeast (Kluyveromyces Lactis) Using a Chemically Defined Medium

Overview

Journal Bioprocess Biosyst Eng

Specialties Biomedical Engineering
Biotechnology

Date 2021 Jan 27

PMID 33502625

Citations 7

Authors

Se-Woong Park

Byung-Ha Kang

Hyeong-Min Lee

Sung-Jun Lee

Han-Seul Kim

Hye-Won Choi

Tae Jung Park

Kwang-Hoon Kong

Affiliations

Soon will be listed here.

Abstract

The sweet-tasting protein brazzein offers considerable potential as a functional sweetener with antioxidant, anti-inflammatory, and anti-allergic properties. Here, we optimized a chemically defined medium to produce secretory recombinant brazzein in Kluyveromyces lactis, with applications in mass production. Compositions of defined media were investigated for two phases of fermentation: the first phase for cell growth, and the second for maximum brazzein secretory production. Secretory brazzein expressed in the optimized defined medium exhibited higher purity than in the complex medium; purification was by ultrafiltration using a molecular weight cutoff, yielding approximately 107 mg L. Moreover, the total media cost in this defined medium system was approximately 11% of that in the optimized complex medium to generate equal amounts of brazzein. Therefore, the K. lactis expression system is useful for mass-producing recombinant brazzein with high purity and yield at low production cost and indicates a promising potential for applications in the food industry.

Citing Articles

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