Human Natural Anti-alpha-galactosyl IgG. II. The Specific Recognition of Alpha (1----3)-linked Galactose Residues

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1985 Aug 1

PMID 2410529

Citations 107

Authors

U Galili

B A Macher

J Buehler

S B SHOHET

Affiliations

Soon will be listed here.

Abstract

A natural IgG antibody (anti-Gal) with alpha-galactosyl binding specificity has been found in large amounts (0.5 - 1.0% of serum IgG) in all individuals tested. It has been purified by affinity chromatography on a column of melibiose-Sepharose. In addition to its affinity for normal and pathological senescent human red cells, the antibody readily interacts with rabbit red blood cell (RRBC) glycolipids with alpha-galactosyl terminal residues. Two types (glycosidic linkages of 1----3 vs. 1----4) of rabbit red cells glycolipids with terminal alpha-galactosyl residues were tested for antibody binding. The antibody specifically bound to glycolipids with Gal alpha 1----3 terminal residues, and treatment of these glycolipids with alpha-galactosidase abolished binding. Hemagglutination inhibition studies with oligosaccharides of known structure also showed that the antibody binds specifically to glycoconjugates with an alpha 1----3 terminal galactose residue. Anti-Gal did not bind to a human B-active glycolipid, indicating that fucose-linked alpha 1----2 to the penultimate galactose prevents anti-Gal binding. The anti-Gal specificity for RRBC glycolipids also paralleled that of the alpha-galactosyl-specific Bandeiraea simplicifolia lectin. The possible reasons for the occurrence of this unique antibody in human serum are discussed.

Citing Articles

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Regeneration in Mice of Injured Skin, Heart, and Spinal Cord by α-Gal Nanoparticles Recapitulates Regeneration in Amphibians.

Galili U, Li J, Schaer G Nanomaterials (Basel). 2024; 14(8).

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Tick bites, IgE to galactose-alpha-1,3-galactose and urticarial or anaphylactic reactions to mammalian meat: The alpha-gal syndrome.

Wilson J, Erickson L, Levin M, Ailsworth S, Commins S, Platts-Mills T Allergy. 2024; 79(6):1440-1454.

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Accelerated Burn Healing in a Mouse Experimental Model Using α-Gal Nanoparticles.

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