Condensin I Associates with Structural and Gene Regulatory Regions in Vertebrate Chromosomes

Overview

Journal Nat Commun

Specialty Biology

Date 2013 Oct 4

PMID 24088984

Citations 50

Authors

Ji Hun Kim

Tao Zhang

Nicholas C Wong

Nadia Davidson

Jovana Maksimovic

Alicia Oshlack

William C Earnshaw

Paul Kalitsis

Damien F Hudson

Affiliations

Soon will be listed here.

Abstract

The condensin complex is essential for correct packaging and segregation of chromosomes during mitosis and meiosis in all eukaryotes. To date, the genome-wide location and the nature of condensin-binding sites have remained elusive in vertebrates. Here we report the genome-wide map of condensin I in chicken DT40 cells. Unexpectedly, we find that condensin I binds predominantly to promoter sequences in mitotic cells. We also find a striking enrichment at both centromeres and telomeres, highlighting the importance of the complex in chromosome segregation. Taken together, the results show that condensin I is largely absent from heterochromatic regions. This map of the condensin I binding sites on the chicken genome reveals that patterns of condensin distribution on chromosomes are conserved from prokaryotes, through yeasts to vertebrates. Thus in three kingdoms of life, condensin is enriched on promoters of actively transcribed genes and at loci important for chromosome segregation.

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