An Improved Selective Culture Medium Enhances the Isolation of Burkholderia Pseudomallei from Contaminated Specimens

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2013 Sep 25

PMID 24062483

Citations 8

Authors

Andrew Goodyear

Linda Strange

Drew A Rholl

Joy Silisouk

David A B Dance

Herbert P Schweizer

Steven Dow

Affiliations

Soon will be listed here.

Abstract

Burkholderia pseudomallei is a Gram-negative environmental bacterium found in tropical climates that causes melioidosis. Culture remains the diagnostic gold standard, but isolation of B. pseudomallei from heavily contaminated sites, such as fecal specimens, can be difficult. We recently reported that B. pseudomallei is capable of infecting the gastrointestinal tract of mice and suggested that the same may be true in humans. Thus, there is a strong need for new culture techniques to allow for efficient detection of B. pseudomallei in fecal and other specimens. We found that the addition of norfloxacin, ampicillin, and polymyxin B to Ashdown's medium (NAP-A) resulted in increased specificity without affecting the growth of 25 B. pseudomallei strains. Furthermore, recovery of B. pseudomallei from human clinical specimens was not affected by the three additional antibiotics. Therefore, we conclude that NAP-A medium provides a new tool for more sensitive isolation of B. pseudomallei from heavily contaminated sites.

Citing Articles

Environmental Factors Associated With Soil Prevalence of the Melioidosis Pathogen : A Longitudinal Seasonal Study From South West India.

Shaw T, Assig K, Tellapragada C, Wagner G, Choudhary M, Gohler A Front Microbiol. 2022; 13:902996.

PMID: 35847064 PMC: 9283100. DOI: 10.3389/fmicb.2022.902996.

Disruption of c-di-GMP Signaling Networks Unlocks Cryptic Expression of Secondary Metabolites during Biofilm Growth in .

Borlee G, Mangalea M, Martin K, Plumley B, Golon S, Borlee B Appl Environ Microbiol. 2022; 88(8):e0243121.

PMID: 35357191 PMC: 9040570. DOI: 10.1128/aem.02431-21.

Human Melioidosis.

Gassiep I, Armstrong M, Norton R Clin Microbiol Rev. 2020; 33(2).

PMID: 32161067 PMC: 7067580. DOI: 10.1128/CMR.00006-19.

Complete Genome Sequence of Pandoraea pnomenusa TF-18, a Multidrug-Resistant Organism Isolated from the Rhizosphere of Rice (Oryza sativa L. subsp. ).

Mangalea M, Luna E, Ziegle J, Chang C, Bosco-Lauth A, Bowen R Microbiol Resour Announc. 2020; 9(1).

PMID: 31896624 PMC: 6940276. DOI: 10.1128/MRA.01008-19.

The cost-effectiveness of the use of selective media for the diagnosis of melioidosis in different settings.

Dance D, Sihalath S, Rith K, Sengdouangphachanh A, Luangraj M, Vongsouvath M PLoS Negl Trop Dis. 2019; 13(7):e0007598.

PMID: 31306412 PMC: 6658006. DOI: 10.1371/journal.pntd.0007598.

References

West T, Myers N, Limmathurotsakul D, Liggitt H, Chantratita N, Peacock S . Pathogenicity of high-dose enteral inoculation of Burkholderia pseudomallei to mice. Am J Trop Med Hyg. 2010; 83(5):1066-9. PMC: 2963971. DOI: 10.4269/ajtmh.2010.10-0306. View

Rholl D, Papp-Wallace K, Tomaras A, Vasil M, Bonomo R, Schweizer H . Molecular Investigations of PenA-mediated β-lactam Resistance in Burkholderia pseudomallei. Front Microbiol. 2011; 2:139. PMC: 3129521. DOI: 10.3389/fmicb.2011.00139. View

Howard K, Inglis T . Novel selective medium for isolation of Burkholderia pseudomallei. J Clin Microbiol. 2003; 41(7):3312-6. PMC: 165331. DOI: 10.1128/JCM.41.7.3312-3316.2003. View

Tuanyok A, Leadem B, Auerbach R, Beckstrom-Sternberg S, Beckstrom-Sternberg J, Mayo M . Genomic islands from five strains of Burkholderia pseudomallei. BMC Genomics. 2008; 9:566. PMC: 2612704. DOI: 10.1186/1471-2164-9-566. View

Eickhoff T, Bennett J, Hayes P, Feeley J . Pseudomonas pseudomallei: susceptibility to chemotherapeutic agents. J Infect Dis. 1970; 121(2):95-102. DOI: 10.1093/infdis/121.2.95. View

Smith M, Wuthiekanun V, Walsh A, White N . Quantitative recovery of Burkholderia pseudomallei from soil in Thailand. Trans R Soc Trop Med Hyg. 1995; 89(5):488-90. DOI: 10.1016/0035-9203(95)90078-0. View

Coenye T, LiPuma J, Henry D, Hoste B, Vandemeulebroecke K, Gillis M . Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients. Int J Syst Evol Microbiol. 2001; 51(Pt 2):271-9. DOI: 10.1099/00207713-51-2-271. View

Jayanetra P, Pipatanagul S, PUNYAGUPTA S, Ratanabanangkoon K, Varavithya W . Pseudomonas pseudomallei: 1. Infection in Thailand. Southeast Asian J Trop Med Public Health. 1974; 5(4):487-91. View

Goodyear A, Bielefeldt-Ohmann H, Schweizer H, Dow S . Persistent gastric colonization with Burkholderia pseudomallei and dissemination from the gastrointestinal tract following mucosal inoculation of mice. PLoS One. 2012; 7(5):e37324. PMC: 3356274. DOI: 10.1371/journal.pone.0037324. View

10.

. In vitro susceptibility of Pseudomonas mallei to antimicrobial agents. Comp Immunol Microbiol Infect Dis. 1989; 12(1-2):5-8. DOI: 10.1016/0147-9571(89)90003-9. View

11.

Brundage W, Thuss Jr C, Walden D . Four fatal cases of melioidosis in U. S. soldiers in Vietnam. Bacteriologic and pathologic characteristics. Am J Trop Med Hyg. 1968; 17(2):183-91. DOI: 10.4269/ajtmh.1968.17.183. View

12.

Deshazer D, Brett P, Carlyon R, Woods D . Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene. J Bacteriol. 1997; 179(7):2116-25. PMC: 178945. DOI: 10.1128/jb.179.7.2116-2125.1997. View

13.

Currie B, Ward L, Cheng A . The epidemiology and clinical spectrum of melioidosis: 540 cases from the 20 year Darwin prospective study. PLoS Negl Trop Dis. 2010; 4(11):e900. PMC: 2994918. DOI: 10.1371/journal.pntd.0000900. View

14.

Holden M, Titball R, Peacock S, Cerdeno-Tarraga A, Atkins T, Crossman L . Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc Natl Acad Sci U S A. 2004; 101(39):14240-5. PMC: 521101. DOI: 10.1073/pnas.0403302101. View

15.

Glass M, Beesley C, Wilkins P, Hoffmaster A . Comparison of four selective media for the isolation of Burkholderia mallei and Burkholderia pseudomallei. Am J Trop Med Hyg. 2009; 80(6):1023-8. View

16.

Loveleena , Chaudhry R, Dhawan B . Melioidosis; the remarkable imitator: recent perspectives. J Assoc Physicians India. 2005; 52:417-20. View

17.

Livermore D, Chau P, Wong A, Leung Y . beta-Lactamase of Pseudomonas pseudomallei and its contribution to antibiotic resistance. J Antimicrob Chemother. 1987; 20(3):313-21. DOI: 10.1093/jac/20.3.313. View

18.

Thibault F, Hernandez E, Vidal D, GIRARDET M, Cavallo J . Antibiotic susceptibility of 65 isolates of Burkholderia pseudomallei and Burkholderia mallei to 35 antimicrobial agents. J Antimicrob Chemother. 2004; 54(6):1134-8. DOI: 10.1093/jac/dkh471. View

19.

Keith K, Oyston P, Crossett B, Fairweather N, Titball R, Walsh T . Functional characterization of OXA-57, a class D beta-lactamase from Burkholderia pseudomallei. Antimicrob Agents Chemother. 2005; 49(4):1639-41. PMC: 1068585. DOI: 10.1128/AAC.49.4.1639-1641.2005. View

20.

Mima T, Schweizer H . The BpeAB-OprB efflux pump of Burkholderia pseudomallei 1026b does not play a role in quorum sensing, virulence factor production, or extrusion of aminoglycosides but is a broad-spectrum drug efflux system. Antimicrob Agents Chemother. 2010; 54(8):3113-20. PMC: 2916348. DOI: 10.1128/AAC.01803-09. View