The Structural Basis of Autotransporter Translocation by TamA

Overview

Journal Nat Struct Mol Biol

Specialties Cell Biology
Molecular Biology

Date 2013 Sep 24

PMID 24056943

Citations 71

Authors

Fabian Gruss

Franziska Zahringer

Roman P Jakob

Bjorn M Burmann

Sebastian Hiller

Timm Maier

Affiliations

Soon will be listed here.

Abstract

TamA is an Escherichia coli Omp85 protein involved in autotransporter biogenesis. It comprises a 16-stranded transmembrane β-barrel and three POTRA domains. The 2.3-Å crystal structure reveals that the TamA barrel is closed at the extracellular face by a conserved lid loop. The C-terminal β-strand of the barrel forms an unusual inward kink, which weakens the lateral barrel wall and creates a gate for substrate access to the lipid bilayer.

Citing Articles

The translocation assembly module (TAM) catalyzes the assembly of bacterial outer membrane proteins .

Wang X, Nyenhuis S, Bernstein H bioRxiv. 2024; .

PMID: 39372782 PMC: 11451606. DOI: 10.1101/2024.06.20.599893.

Leptospira interrogans encodes a canonical BamA and three novel noNterm Omp85 outer membrane protein paralogs.

Bettin E, Grassmann A, Dellagostin O, Gogarten J, Caimano M Sci Rep. 2024; 14(1):19958.

PMID: 39198480 PMC: 11358297. DOI: 10.1038/s41598-024-67772-6.

The translocation assembly module (TAM) catalyzes the assembly of bacterial outer membrane proteins in vitro.

Wang X, Nyenhuis S, Bernstein H Nat Commun. 2024; 15(1):7246.

PMID: 39174534 PMC: 11341756. DOI: 10.1038/s41467-024-51628-8.

POTRA domains of the TamA insertase interact with the outer membrane and modulate membrane properties.

Mellouk A, Jaouen P, Ruel L, Le M, Martini C, Moraes T Proc Natl Acad Sci U S A. 2024; 121(28):e2402543121.

PMID: 38959031 PMC: 11252910. DOI: 10.1073/pnas.2402543121.

The TAM, a Translocation and Assembly Module for protein assembly and potential conduit for phospholipid transfer.

Goh K, Stubenrauch C, Lithgow T EMBO Rep. 2024; 25(4):1711-1720.

PMID: 38467907 PMC: 11014939. DOI: 10.1038/s44319-024-00111-y.

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