Cloning of an Interleukin-3 Receptor Gene: a Member of a Distinct Receptor Gene Family

Overview

Journal Science

Specialty Science

Date 1990 Jan 19

PMID 2404337

Citations 86

Authors

N Itoh

S Yonehara

J Schreurs

D M Gorman

K Maruyama

A Ishii

I YAHARA

K Arai

A Miyajima

Affiliations

Soon will be listed here.

Abstract

Interleukin-3 (IL-3) binds to its receptor with high and low affinities, induces tyrosine phosphorylation, and promotes the proliferation and differentiation of hematopoietic cells. A binding component of the IL-3 receptor was cloned. Fibroblasts transfected with the complementary DNA bound IL-3 with a low affinity [dissociation constant (Kd) of 17.9 +/- 3.6 nM]. No consensus sequence for a tyrosine kinase was present in the cytoplasmic domain. Thus, additional components are required for a functional high affinity IL-3 receptor. A sequence comparison of the IL-3 receptor with other cytokine receptors (erythropoietin, IL-4, IL-6, and the beta chain IL-2 receptor) revealed a common motif of a distinct receptor gene family.

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